›› 2007, Vol. 34 ›› Issue (9): 47-49.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequence Analysis of Partial Fragment of Prolactin Receptor Gene in Goats

CHU Mingxing1, ZHANG Genxi2, LIU Rongzhi3, WANG Jinyu2, FANG Li1, YE Sucheng1, LIU Xueyun4,MA Mingde4   

  1. 1.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China； 2.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 3.China Association of Agricultrual Science Societies,Beijing 100026,China; 4.Animal Husbandry Bureau of Jiaxiang County in Shandong Province,Jiaxiang 272400,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20

Abstract: The 892 bp fragment （97 bp of part of exon 8 (the full length of exon 8 was 100 bp), 683 bp of intron 8, 70 bp of exon 9 and 42 bp of part of intron 9）of prolactin receptor (PRLR) gene was amplified successfully in Jining Grey goats by PCR and cloned into pGEM-T Easy vector. The positive clones were further identified by PCR analysis. The nucleotide sequence was detected and the peptide sequence of this fragment was deduced. This sequence shared 99.4 %, 97.01%, 89.22%, 89.22%, 88.02% nucleotide homology with the published mRNA of PRLR gene of sheep, cow, human, rat and mouse separately, and the amino acid homology was 100%, 94.55%, 81.88%, 81.82%, 83.64% separately.

Key words: goat; prolactin receptor gene; cloning; sequence analysis

CLC Number: