›› 2007, Vol. 34 ›› Issue (6): 79-81.

• 疾病防治 • Previous Articles     Next Articles

Establishment of the Method of Detecting the Residues of DFLX by Indirect Competitive Enzyme-linked Immunosorbent Assay

LI Zhenhua1,2, SIKANDAER Maihemuti1,WEI Dong2,3,ZHANG Naisheng2
   

  1. 1.College of Animal Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052，China； 2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062,China； 3.Department of Veterinary Medicine, North Hebei University, Zhangjiakou 075000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-20 Published:2007-06-20

Abstract: DFLX hapten was coupled to BSA and OVA, DFLX-OVA was coating antigen, DFLX-BSA was immunity antigen. The indirect competitive ELISA for DFLX was developed. The result of experiment that the optimal concentration of the coating antigen, polyclonal antibody against DFLX and sheep anti-rabbit IgG were 1.25 μg/L, 1∶10000 and 1∶4000 respectively. Quantization of the DFLX was linear from 0.1~10 ng/L. with the detection limit being 0.2 ng/l. The coefficients of variation of intra-assay and inter-assay were 3.81%and 6.25% respectively. Then, the regression equation was y=0.7975-0.125x（R2=0.989）and there was a standard curve. So indirect competitive enzyme-linked immunosorbent assay was established to detect rapidly the residues of DFLX in food of animal origin.

Key words: danofloxacin(DFLX); residue detection; indirect competitive ELISA

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