二氟沙星残留检测的Ci-ELISA方法建立

›› 2007, Vol. 34 ›› Issue (10): 127-129.

• 公共卫生 • Previous Articles Next Articles

EstabLishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Difloxacin

PAN Xiaocheng1,QI Kezong2,ZHU Liangqiang3,ZHAO Rui hong1

1.Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China;2.Anhui Agricultural University, Hefei 230036,China;3.Anhui Veterinary Station,Hefei 230022,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

Abstract

Abstract: The CiELISA method for the detection of difloxacin were established with the polyclonal antibodies against difloxacin. The regression equation, the 50% inhibition concentration(IC50), the limits of detect (LOD) and the linear range about the standard curve of difloxacin were y=-0.223x+0.9631(r=0.9929), 119.21ng/ml，1.14 ng/ml and 1.14~10000 ng/ml, respectively. The average coefficients of variation for the CiELISA method were 4.83% in intraassay and 10.69% in interassay. The recoveries in chicken ranged from 81.24% to 90.64%.

Key words:  difloxacin; CiELISA; residue; chicken

CLC Number:

S859.84

PAN Xiaocheng;QI Kezong;ZHU Liangqiang;ZHAO Rui hong. EstabLishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Difloxacin[J]. , 2007, 34(10): 127-129.

[1]	DU Yuanjun, DUAN Guochao, LIU Wei, LI Dapeng, HAN Haixia, LEI Qiuxia, ZHOU Yan, LIU Jie, WANG Jie, CAO Dingguo, LI Fuwei, CHEN Fu. Effect of Translucent on Egg Quality and Its Relationship with Intestinal Function of Laying Hen [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2305-2311.
[2]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[3]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[4]	WU Jingxuan, SUN Zheng, MA Xuelian, ZHANG Liyang, LIAO Xiudong, LUO Xugang, LI Sufen, LYU Lin. A Survey on Magnesium Contents in Feed Resources for Livestock and Poultry in China [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1888-1898.
[5]	JIANG Shanyi, ZHOU Bijun, WEN Ming, WANG Kaigong. Prevention of Traditional Chinese Medicine with Probiotics on Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2092-2102.
[6]	LIU Xin, CHEN Qun, QIU Yulang, LI Lin, HOU Guoxi, YAN Xiaogang, LI Zhonghe, CHEN Long, GAO Xingai, LIU Dongmei, ZHAI Di, YAN Qiuliang. Research Progress on the Production Mechanism and Control Technology of Chicken Manure Composting Nitrogen-containing and Sulfur-containing Waste Gases [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2166-2174.
[7]	WU Zhi, ZHANG Cong, YUAN Huisha, DONG Hongyan, DAI Lixia, JIA Luqi, WU Shuang, ZHU Shanyuan. Isolation,Identification,Virulence and Drug Resistance of Salmonella from Yellow Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1695-1706.
[8]	HUANG Xiaoqian, YUAN Junzhao, LIU Jinhe, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Protective Effect of Honokiol on Cadmium-induced Renal Injury in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1007-1015.
[9]	LI Xinran, ZHANG Qingyan, ZHA Weiwei, ZHU Miao. Bioinformatics Analysis and Expression of Wnt Gene Family in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 15-25.
[10]	XIE Jingjing, REN Mengting, FAN Jiahui, JIAO Yaru, HAN Zongxi, MA Deying. Study on Immune Response of Infectious Bronchitis Virus to Chickens and Antiviral Effect of Recombinant Avian Beta-defensins 11 and 12 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 246-259.
[11]	ZHANG Hao, LIU Yixuan, ZHU Huiyuan, ZHANG Pengwei, LEI Yanru, GAO Chaoqun, LI Donghua, KANG Xiangtao. Bioinformatics Analysis of Differential Genes of Skin Feather Follicles in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3273-3282.
[12]	GAO Xiaomin, ZHOU Shujian, CHEN Chen, JIN Jing, HU Cai, ZHANG Chen, ZUO Qisheng, ZHANG Yani, CHEN Guohong, LI Bichun. Regulation of STAT1 and Histone Acetylation Modification on lncRNA-BMP4 Transcription in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3321-3332.
[13]	GAO Yefan, SONG Hanan, WANG Yunan, WU Yue, NIU Ruili, ZONG Xianchun, GUAN Weijun. Study on the Isolation, Culture and Differentiation Potential of Limbal Stem Cells from Beijing You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3372-3381.
[14]	WANG Xuefang, GUI Rong, CHEN Gongyi, WANG Wei, ZHANG Hongying, WANG Xuebing, LI Xiao. Clinical Study of Lianhuachaiqin Soluble Powder on Chicken Syndrome of Wind-heat Invading Lung [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3610-3621.
[15]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.

EstabLishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Difloxacin

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments