China Animal Husbandry & Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (6): 2865-2873.doi: 10.16431/j.cnki.1671-7236.2025.06.038

• Basic Veterinary Medicine • Previous Articles     Next Articles

Effects of Isorhapontigenin on Alleviating Oxidative Damage of IPEC-J2 Cells

ZHU Longlong1,2, CAI Long2, LIU Zeqing2,3, SONG Yanchen2,4, WU Qiujue1, WANG Jing2,5   

  1. 1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China;
    2. Institute of Animal Science and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    3. College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    4. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    5. Joint Laboratory of Animal Science, Beijing Academy of Agriculture and Forestry Sciences-Oklahoma State University, Beijing 100097, China
  • Received:2024-09-08 Published:2025-05-27

PDF (PC)

5

Abstract: 【Objective】 The objective of this study was to investigate the alleviating effect of isorhapontigenin (ISO) on oxidative damage in porcine intestinal epithelial cells (IPEC-J2),so as to provide a theoretical reference for the application of ISO as an antioxidant in animal production.【Method】 IPEC-J2 cells were stimulated with hydrogen peroxide (H2O2) to establish the oxidative stress model,and the optimal working concentration of ISO was determined by the cell viability.The contents of malondialdehyde (MDA),reactive oxygen species (ROS) and 8-hydroxy-deoxyguanosine (8-OHDG),the activities of catalase (CAT),total superoxide dismutase (T-SOD),and total antioxidant capacity (T-AOC) in the cells were determined by the corresponding commercial kit.Real-time quantitative PCR was used to detect the relative expression levels of antioxidase genes (SOD-1, CAT, GSH-Px, HO-1 and NQO-1),inflammatory genes (IL-1β, IL-12 and TNF-α),and host defense peptide genes (pBD1， pBD2 and pBD3) in IPEC-J2 cells.【Result】 Pretreatment of cells with 5,20 and 40 μmol/L ISO for 24 h could alleviate the decrease of cell survival caused by H2O2(P＜0.05 or P＜0.01),particularly,the 20 μmol/L ISO group had the best effect among the all groups.ISO pretreatment could reduce the elevation of the levels of MDA,ROS and 8-OHDG in IPEC-J2 cells induced by H2O2 (P＜0.05 or P＜0.01) and increase the relative expression levels of GSH-Px, SOD1,CAT and NQO-1 genes and CAT activity (P＜0.01).In addition,compared with H2O2 group,ISO pretreatment could decrease the relative expression levels of IL-1β and IL-12 genes,and increase the relative expression levels of pBD1,pBD and pBD3 genes (P＜0.05).【Conclusion】 ISO could reduce the pruduction of stress products in cells,improve the expression of antioxidant enzyme genes and activity of antioxidant enzymes,and regulate the expression of inflammation-related genes,thus alleviating the oxidative damage of IPEC-J2 cells caused by H2O2.Under the conditions of this experiment,the recommended ISO concentration was 20 μmol/L.

Key words: isorhapontigenin; oxidative damage; antioxidant; IPEC-J2 cells

CLC Number: