UPLC-Q-TOF-MS结合网络药理学探讨威灵仙水提物抗炎的药效物质及作用机制

doi:10.16431/j.cnki.1671-7236.2025.06.037

Abstract

Abstract: 【Objective】 This study aimed to analyze the main chemical components of Clematidis Radix decoction by ultra performance liquid chromatography with time-of-flight mass spectrometry (UPLC-Q-TOF-MS),and verify its anti-inflammatory mechanism by combining network pharmacology and cellular experiments.【Method】 The chemical composition of Clematidis Radix decoction was analyzed and identified by UPLC-Q-TOF-MS,the main components and corresponding targets were predicted by network pharmacology method,and the "component-target-pathway" network was constructed.The molecular docking between the main active ingredients and the core targets was carried out by AutoDock software to verify the binding ability between the core components and the key targets.The inflammation model of RAW264.7 cells was constructed by lipopolysaccharide (LPS) induction,and the core target and pathway were verified.【Result】 A total of 19 components were identified from Clematidis Radix decoction,including 8 phenolic acids and 11 saponins.Network pharmacological analysis screened 5 main active ingredients mainly modulating the PI3K/Akt/STAT3 signaling pathway mediated by core targets including phosphatidylinositol 3-kinase catalytic subunit α (PIK3CA),signal transduction and transcriptional activator 3 (STAT3),phosphatidylinositol 3-kinase catalytic subunit β (PIK3CB),and tumor necrosis factor (TNF) to exert anti-inflammatory effects.ELISA test results showed that compared with control group,the contents of inflammatory cytokines TNF-α,interleukin-6 (IL-6) and IL-1β in model group were significantly increased (P＜0.05).Compared with model group,the contents of TNF-α,IL-6 and IL-1β in each dose group of Clematidis Radix decoction were significantly decreased (P＜0.05). Western blotting results showed that compared with control group,the protein expressions of PI3K,p-PI3K,Akt and p-Akt in model group were significantly up-regulated (P＜0.05).Compared with model group,the expression levels of p-PI3K and p-Akt were significantly decreased in each dose group of Clematidis Radix decoction (P＜0.05). 【Conclusion】 Clematidis Radix could exert anti-inflammatory effects through multi-component,multi-target and multi-pathway,and the target was related to PI3K/Akt signaling pathway.The results of this study provided a reference for further study of the pharmacological material basis and pharmacological activity of Clematidis Radix.

Key words: Clematidis Radix; UPLC-Q-TOF-MS; network pharmacology; anti-inflammatory; inflammatory factor

CLC Number:

S853.74

CHEN Haiyan, ZHANG Jingzheng, XU Chenxin, LIU Yuanfen, ZHOU Yongmei, HAN Jin. Exploration of the Pharmacodynamic Substances of Anti-inflammatory and Mechanism of Clematidis Radix Decoction Using UPLC-Q-TOF-MS Combined with Network Pharmacology[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2851-2864.

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Exploration of the Pharmacodynamic Substances of Anti-inflammatory and Mechanism of Clematidis Radix Decoction Using UPLC-Q-TOF-MS Combined with Network Pharmacology

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