黄淮肉羊IGF1基因序列特征、组织表达及其与产肉性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2024.12.025

Abstract

Abstract: 【Objective】 This study was aimed to research the sequence characteristics and tissue expression patterns of insulin like growth factor 1 (IGF1) gene in Huanghuai sheep,and conduct correlation analysis between the expression of IGF1 gene in longissimus dorsi muscle and meat production traits.【Method】 The 9 male Huanghuai sheep at the ages of 3,9,and 18 months each were selected.The meat production performance were determined and semitendinosus,quadriceps femoris,triceps brachii,longissimus dorsi muscle,subcutaneous fat,rumen,kidney,spleen,liver,testis and small intestine tissue samples were collected after slaughter.the IGF1 gene of Huanghuai sheep was cloned and sequenced，and analyzed by bioinformatics analysis.The mRNA levels of IGF1 gene in various tissues at 9-month-old and in the longissimus dorsi muscle at different age stages were detected by Real-time quantitative PCR,and the correlation between IGF1 expression and meat production traits were analyzed through Pearson correlation coefficient.【Result】 The CDS region size of IGF1 gene in Huanghuai sheep was 465 bp,encoding 154 amino acids,indicating the closest genetic relationship with Capra hircus.IGF1 protein of Huanghuai sheep was a hydrophilic and unstable protein with no transmembrane structure.It contained signal peptides and had 29 potential phosphorylation sites.The secondary structure of IGF1 protein in Huanghuai sheep mainly contained random coil (54.55%) and alpha helix (29.87%)，and the predicted results of the tertiary structure were consistent with the secondary structure.There were interactions between IGF1 and IGF1R,IGFBP3,IGF2 and other proteins.The results of Real-time quantitative PCR showed that IGF1 gene was widely expressed in the tissues of Huanghuai sheep,with the highest expression in liver and the lowest expression in triceps brachii.The expression level of IGF1 gene was highest in the longissimus dorsi muscle of 3-month-old Huanghuai sheep,significantly higher than that of 9-month-old and 18-month-old sheep (P＜0.05),while the expression of 9-month-old sheep was significantly lower than that of 18-month-old sheep (P＜0.05).The correlation analysis results showed that the expression of IGF1 gene was not significantly correlated with growth traits and meat quality indicators (P＞0.05).There was a significant or extremely significant positive correlation between body height,body length,chest girth,cannon circumference,body weight,and carcass weight (P＜0.05 or P＜0.01).There was also a significant or extremely significant positive correlation between dressing percentage,meat color,marble texture,and loin muscle area (P＜0.05 or P＜0.01).【Conclusion】 The CDS sequence of IGF1 gene in Huanghuai sheep was successfully cloned.The IGF1 protein was the hydrophilic and unstable alkaline protein,mainly expressed in the liver,and the general trend in the longissimus dorsi muscle muscle were first decreasing and then rising with age increasing,and was not significantly correlated with growth and development and meat quality indicators.These results provided a reference for further exploring the role of IGF1 gene in muscle development and meat quality regulation in Huanghuai sheep.

Key words: Huanghuai sheep; IGF1 gene; tissue expression; correlation

CLC Number:

S826

LI Jun, SONG Mengke, GAO Fuxian, HAN Wanli, HAN Haoyuan, SHI Huibin, QUAN Kai. Sequence Characteristics,Tissue Expression of IGF1 Gene and Correlation with Production Trait in Huanghuai Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(12): 5380-5391.

References

[1] 赵海东，邬明丽，陈平博，等.IGF2基因表达调控及其遗传变异在动物生长发育中的研究进展[J].中国畜牧兽医，2020，47(6)：1844-1852.ZHAO H D，WU M L，CHEN P B，et al.Research progress of IGF2 gene expression regulation and its genetic variation in animal growth and development[J].China Animal Husbandry & Veterinary Medicine，2020，47(6)：1844-1852.(in Chinese)
[2] 宋林锦，许厚强，李永，等.关岭牛IGF1基因干扰载体构建及其对成肌细胞的影响[J].农业生物技术学报，2022，30(5)：896-907.SONG L J，XU H Q，LI Y，et al.Construction of IGF1 gene interference vector in guanling cattle (Bos taurus) and its effect on myogenic cells[J].Chinese Journal of Agricultural Biotechnology，2022，30(5)：896-907.(in Chinese)
[3] 王冬，李发弟，王维民，等.绵羊IGF-Ⅰ和IGF-ⅠR基因特征分析及其在肌肉组织中的表达差异[J].甘肃农业大学学报，2015，50(2)：6-15.WANG D，LI F D，WANG W M，et al.Bioinformatics analysis on IGF-Ⅰand IGF-1R genes and their different expression in muscle tissue of sheep[J].Journal of Gansu Agricultural University，2015，50(2)：6-15.(in Chinese)
[4] 闫云峰，杨华，杨永林，等.日粮不同蛋白质水平对绵羊IGF-1和GH分泌及基因表达的影响[J].畜牧兽医学报，2015，46(1)：85-95.YAN Y F，YANG H，YANG Y L，et al.Effects of different dietary protein levels on the secretion and mRNA expression of IGF-1 and GH in sheep[J].Acta Veterinaria et Zootechnica Sinica，2015，46(1)：85-95.(in Chinese)
[5] 唐文.NAC对影响山羊产羔性状的IGF1、IGFBP1基因表达调控研究[D].贵阳：贵州大学，2021.TANG W.Studies on NAC regulation of IGF1 and IGFBP1 genes that influence lambing traits of goat[D].Guiyang：Guizhou University，2021.(in Chinese)
[6] 朱晓锋，许厚强，陈伟，等.从江香猪IGF-1和IGF-2基因的克隆、表达及生物信息学分析[J].农业生物技术学报，2019，27(8)：1382-1391.ZHU X F，XU H Q，CHEN W，et al.Cloning, expression and bioinformatics analysis of IGF-1 and IGF-2 gene Congjiangxiang pigs (Sus scrofa)[J].Journal of Agricultural Biotechnology，2019，27(8)：1382-1391.(in Chinese)
[7] ISLAM K K，VINSKY M, CREWS R E，et al.Association analyses of a SNP in the promoter of IGF1 with fat deposition and carcass merit traits in hybrid, Angus and Charolais beef cattle[J].Animal Genetics，2009，40(5)：766-769.
[8] WANG W J，GUO Y Q，XIE K J，et al.A functional variant in the promoter region of IGF1 gene is associated with chicken abdominal fat deposition[J].Domestic Animal Endocrinology，2021，75：106584-106593.
[9] 束婧婷，姬改革，单艳菊，等.IGF1-PI3K-Akt信号通路相关基因在黄羽肉鸡肌肉和肝脏中的表达[J].中国农业科学，2021，54(9)：2027-2038.SHU J T，JI G G，SHAN Y J，et al.Expression analysis of IGF1-PI3K-Akt-dependent pathway genes in skeletal muscle and liver tissue of yellow feather broilers[J].Scientia Agricultura Sinica，2021，54(9)：2027-2038.(in Chinese)
[10] DING N，TIAN D，LI X，et al.Genetic polymorphisms of IGF1 and IGF1R genes and their effects on growth traits in Hulun Buir sheep[J].Genes，2022，13(4)：666-678.
[11] 肖超能，翁吉梅，李维，等.家兔IGF1基因多态性与生长性状的关联分析[J].中国畜牧兽医，2017，44(8)：2378-2385.XIAO C N，WENG J M，LI W，et al.Association analysis between polymorphism of IGF1 gene and growth traits in rabbit[J].China Animal Husbandry & Veterinary Medicine，2017，44(8)：2378-2385.(in Chinese)
[12] RENAVILLE R，HAMMADI M，PORTETELLE D.Role of the somatotropic axis in the mammalian metabolism[J].Domestic Animal Endocrinology，2002，23(1-2)：351-360.
[13] MEIRA A N，MONTENEGRO H，COUTINHO L L, et al.Single nucleotide polymorphisms in the growth hormone and IGF type-1(IGF1) genes associated with carcass traits in Santa Ines sheep[J].Animal，2019，13(3)：460-468.
[14] 李沐，李婷，孟星宇，等.梅花鹿IGF1全长cDNA的克隆及在鹿茸组织的表达[J].畜牧与兽医，2012，44(S1)：214.LI M，LI T，MENG X Y，et al.Cloning of the full-length cDNA of sika deer IGF1 and its expression in deer antler tissues[J].Animal Husbandry and Veterinary Medicine，2012，44(S1)：214.(in Chinese)
[15] 宋旭婷，徐雪，张嘉楠，等.绵羊IGF-Ⅰ基因剪接异构体克隆及生物信息学分析[J].中国科技论文，2017，12(12)：1399-1406.SONG X T，XU X，ZHANG J N，et al.Cloning and bioinformatics analysis of splice isoforms of sheep IGF-Ⅰ gene[J].China Sciencepaper，2017，12(12)：1399-1406.(in Chinese)
[16] BLYTH A J，KIRK N S，FORBES B E.Understanding IGF-Ⅱ action through insights into receptor binding and activation[J].Cells，2020，9(10)：2276-2289.
[17] 张雨婷，王翠，刘毅，等.浙东白鹅IGF1基因克隆、组织表达及与体重性状关联分析[J].中国家禽，2023，45(5)：24-30.ZHANG Y T，WANG C，LIU Y，et al.Cloning, tissue expression and association analysis with body weight IGF1 gene in Zhedong White goose[J].China Poultry，2023，45(5)：24-30.(in Chinese)
[18] TAKAHASHI Y.The role of growth hormone and insulin-like growth factor-Ⅰ in the liver[J].International Journal Molecular Sciences，2017，18(7)：1447-1460.
[19] 赵茹茜，贾斌，剡根强，等.绵羊皮肤中生长激素受体、胰岛素样生长因子1和胰岛素样生长因子1受体基因表达的发育性变化及品种特点[A].动物生理生化学分会第八次学术会议暨全国反刍动物营养生理生化第三次学术研讨会[C].2004.ZHAO R Q，JIA B，YAN G Q，et al.Developmental changes and breed characteristics of growth hormone receptor, insulin-like growth factor 1 and insulin-like growth factor 1 receptor gene expression in sheep skin[A].The 8th Academic Conference of the Animal Physiology and Biochemistry Branch and the 3rd National Symposium on Nutritional Physiology and Biochemistry of Ruminant Animals[C].2004.(in Chinese)
[20] 李文杨，刘远，吴贤锋，等.山羊IGF-1基因的骨骼肌表达特性及其SNPs与生长性状的关联分析[J].农业生物技术学报，2019，27(12)：2188-2197.LI W Y，LIU Y，WU X F，et al.Skeletal muscle expression characteristics of IGF-1 gene and tts SNPs association with growth traits in goat(Capra hircus)[J].Journal of Agricultural Biotechnology，2019，27(12)：2188-2197.(in Chinese)
[21] GROCHOWSKA E，LISIAK D，AKRAM M Z，et al.Association of a polymorphism in exon 3 of the IGF1R gene with growth，body size，slaughter and meat quality traits in Colored Polish Merino sheep[J].Meat Science，2021，172：108314-108321.
[22] 强浩，梁鹏，孟科，等.绵羊IGF1R基因多态性及其与生长性状的关联分析[J].中国畜牧兽医，2022，49(5)：1765-1785.QIANG H，LIANG P，MENG K，et al.Polymorphism of IGF1R gene and its association analysis with growth traits in sheep[J].China Animal Husbandry & Veterinary Medicine，2022，49(5)：1765-1785.(in Chinese)
[23] FROST A，PROSKURA W S，BARNIK J，et al.Variability within IGF1 and its linkage to body size and predisposition to hip dysplasia in dogs[J].The Indian Journal of Animal Sciences，2015，85(4)：411-413.
[24] 杨烨城，黄秋艳，辛海云，等.藏猪和杜藏猪生长、肉质性状及脂肪沉积相关基因的表达差异分析[J].南方农业学报，2022，53(4)：926-934.YANG Y C，HUANG Q Y，XIN H Y，et al.Analysis of differences in growth, meat quality traits and expression of gene related to fat deposition between Tibetan and Duzang pigs[J].Southern Journal of Agriculture，2022，53(4)：926-934.(in Chinese)
[25] 张斌，相德才，赵智勇，等.IGFs和MSTN基因表达量与种猪生长性状的相关性研究[J].云南农业大学学报(自然科学)，2019，34(2)：263-270.ZHANG B，XIANG D C，ZHAO Z Y，et al.Correlation analysis on expression of IGFs and MSTN genes and growth traits in breeding pigs[J].Journal of Yunnan Agricultural University (Natural Science)，2019，34(2)：263-270.(in Chinese)

Sequence Characteristics,Tissue Expression of IGF1 Gene and Correlation with Production Trait in Huanghuai Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHOU Guangxian, TAN Jiayong, YANG Jian, LIU Yanfen, GAN Shangquan, ZHAO Zhihui, KANG Danju. Polymorphism of TBX15 Gene and Its Association Analysis with Body Size Traits in Leizhou Goats [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 3930-3938.
[2]	HUANG Yuechuan, ZHANG Hailiang, XU Wei, HAN Liyun, ZHOU Jiamin, MA Liqin, WEN Wan, WANG Yachun. Genetic Parameters for Conformation Traits of Dairy Cattle in Ningxia [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2908-2922.
[3]	JIANG Lei, WANG Yuxin, WAN Yuan, ZAN Linsen, WANG Hongbao. Bioinformatics and Expression Characteristics Analysis of MGP Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2261-2272.
[4]	SUN Shiyu, LI Jinlan, XING Jiani, DONG Yaohui, LI Yanyan, WANG Youli, LIN Yaqiu. Cloning,Bioinformatics Analysis and Spatiotemporal Expression of CKMT2 Gene in Jianzhou Big-eared Goats [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2342-2353.
[5]	HUANG Liqing, DUAN Anqin, ZHENG Haiying, YANG Chunyan, SHANG Jianghua. Sequence Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of SFRP1 Gene in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 1807-1818.
[6]	YAN Zunqiang, LIANG Yuhao, SONG Kelin, GUN Shuangbao, WANG Pengfei. Cloning,Bioinformatics Analysis and Tissue Expression of SIRT3 Gene in Hezuo Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1390-1399.
[7]	MA Rong, LA Yongfu, BAO Pengjia, GUO Xian, LU Jianwei, ZHA Lao, ZHAO Xue, LIANG Chunnian, CHENG Shuru. Cloning,Bioinformatics Analysis and Tissue Expression of MYL9 Gene in Meiren Yak [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1410-1419.
[8]	HUANG Yongpeng, RAN Xueqin, NIU Xi, LI Sheng, HUANG Shihui, WANG Jiafu. Gene Expression Change and Its Correlation Analysis with Meat Quality During Postmortem Pork Aging [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1573-1581.
[9]	SU Yanfang, NIU Naiqi, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Polymorphisms of LPL and NUDT3 Genes and Its Association Analysis with Meat Quality Traits in Beijing Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1111-1121.
[10]	HUANG Yuechuan, ZHANG Hailiang, LIU Zhanpeng, ZHAO Shanjiang, SUN Wei, LI Xihe, WANG Lin, ZHU Huabin, HUANG Xixia, WANG Yachun. Analysis of the Population Characteristics and Influencing Factors of Superovulation Response Traits in Northern Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 4851-4859.
[11]	LIU Yu, ZHANG Linlin, FANG Yi, ZHANG Jinlong, LI Yihai, ZHONG Rongzhen, SHENG Jiahai, HE Yongxiang, GUO Xiaofei, ZHANG Xiaosheng. Polymorphism of STAT5a Gene Intron 10 and Its Association with Lactation Traits in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3680-3687.
[12]	BI Yazhen, SHANG Mingyu, XIONG Jinke, HU Wenping, HE Jianning, ZHANG Li. Polymorphism of SRP68 Gene and Its Association with Growth Traits in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3189-3198.
[13]	XING Shanshan, LI Xin, YU Lei, XU Huifen, CAI Hanfang, LI Ming. Cloning, Bioinformatics Analysis and Tissue Expression of SOX6 Gene in Xianan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2651-2660.
[14]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[15]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.