沙门菌感染小鼠氧化应激模型的建立及其分子机制研究

doi:10.16431/j.cnki.1671-7236.2021.10.036

Abstract

Abstract: The study was aimed to establish the oxidative stress model of Kunming mouse induced by Salmonella Typhimurium, and explore its molecular mechanism. 18 mice were randomly divided into 3 groups with 6 mice in each group. Normal saline (control group), low dose Salmonella solution (group 1) and high dose Salmonella solution (group 2) were given by gavage respectively. Mice were dissected and samples were collected after gavage for 24 h. Serum oxidative stress indexes were detected. HE sections of liver, duodenum, jejunum and ileum were observed and scored. The mRNA expression of liver and duodenal antioxidant enzymes, key proteins in the antioxidant signal pathway, and tight junction proteins were detected. The results showed that:Compared with the control group, superoxide dismutase (SOD) and glutathione peroxidase (GPx1) activity in the serum of the group 2 decreased significantly or extremely significantly (P<0.05;P<0.1), while malondialdehyde (MDA) increased extremely significantly (P<0.01). The duodenal villi were broken and dropped off, and ulceronecrotic of the intestinal lamina propria, and the liver mainly showed inflammatory cell infiltration, hepatic cord disorders and punctate necrosis in groups 1 and 2. Compared with the control group, the expression of manganese superoxide dismutase (SOD2), GPx1, heme oxygenase-1 (HO-1), sequestosome 1 (p62) and nuclear factor E2-related factor 2 (Nrf2) mRNA decreased significantly or extremely significantly in duodenum and liver (P<0.05;P<0.01), while the expression of ZO-1 and OCLN mRNA in duodenal, and the expression of ZO-1 and claudin-8 (CLDN8) mRNA in liver decreased extremely significantly (P<0.01) in group 2. The above results showed that the oxidative stress model of mice was successfully established by high dose Salmonella solution, and it was found that high dose Salmonella solution might inhibit the expression of antioxidant enzymes in duodenum and liver by reducing the transcription of p62 and Nrf2, resulting in the damage of cell barrier function in duodenum and liver.

Key words: Salmonella; oxidative stress; tight junctions

CLC Number:

S852.61

LIU Xuejiao, LI Haihua, WANG Yimeng, SHANG Zhiyuan, WU Yue, QIAO Jiayun. Establishment of Oxidative Stress Model in Mice Infected with Salmonella and Its Molecular Mechanism[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3834-3844.

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Establishment of Oxidative Stress Model in Mice Infected with Salmonella and Its Molecular Mechanism

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