虹鳟酪氨酸酶基因的生物信息学分析及其在不同发育阶段和组织中的表达分析

doi:10.16431/j.cnki.1671-7236.2021.05.002

Abstract

Abstract: Tyrosinase (Tyr) gene plays a key role in the process of regulating animal pigment synthesis.In order to understand the relationship between tyr gene and body colour variation of rainbow trout,in this study,the protein sequence analysis of tyr-1 and tyr-2 genes in rainbow trout was performed,the expression of 19 different development stages and 13 different tissues was determined by Real-time quantitative PCR in wild-type rainbow trout (WT) and yellow mutant rainbow trout (YT).Protein sequence analysis results showed that Tyr-1 and Tyr-2 proteins were both hydrophilic proteins and the structure was mainly random coil and alpha helix;Real-time quantitative PCR results showed that tyr-1 gene was expressed in WT and YT embryos at all stages.In WT,the expression of tyr-1 gene was the highest in the fertilized-stage,followed by the multi-cell stage,which was significantly higher than other stages (P<0.05),In YT,the expression of tyr-1 gene expression was the highest at the 16-cell,which significantly higher than other periods (P<0.05);tyr-2 gene was expressed from the WT blastula stage and the YT gastrula stage,the expression level reached the highest in the heartbeating stage,which was significantly higher than other periods (P<0.05).The expression levels of tyr-1 and tyr-2 genes were the highest in WT at 5 days post hatch and YT at 3 months post hatch.The expression levels of tyr-1 and tyr-2 genes in WT were higher than those in YT at embryonic stage and generally lower than those in YT during post hatch.In adult fish tissues,tyr-1 gene was highly expressed in skin,eyes,kidney,liver and other tissues,the tyr-2 gene was highly expressed in skin and eyes,and it was rarely expressed in other tissues.The above results indicated that tyr-1 and tyr-2 genes had a certain correlation with the body color variation of rainbow trout,which provided a theoretical basis for in-depth elucidation of the body color variation mechanism and body color genetic improvement of rainbow trout.

Key words: rainbow trout; body colour; tyr gene; bioinformatics analysis; expression analysis

CLC Number:

Q786

WANG Xiaolan, WU Shenji, HUANG Jinqiang, LI Yongjuan, PAN Yucai, ZHANG Qian. Bioinformatics and Expression Analysis of Tyrosinase Gene at the Different Stages and Tissues of Rainbow Trout (Oncorhynchus mykiss)[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1516-1524.

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Bioinformatics and Expression Analysis of Tyrosinase Gene at the Different Stages and Tissues of Rainbow Trout (Oncorhynchus mykiss)

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