山羊ACSS2基因的克隆、序列分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2020.02.003

Abstract

Abstract: This study was aimed to clone acetyl-CoA synthetase 2(ACSS2) gene in goats,perform bioinformatics analysis,and detect its mRNA expression level in mammary gland during different lactation stages in goats.Mammary gland tissue RNA in goats was used as a template,and the complete CDS region of ACSS2 gene in goats was amplified and cloned.The sequencing result was analyzed by bioinformatics,and the expression of ACSS2 gene in mammary gland tissues during different lactation stages in goats were analyzed.The results showed that the total CDS region sequence of ACSS2 gene in goats was 2 106 bp,which encoded 701 amino acids.The homology of ACSS2 gene in goats with Bos taurus,Equus caballus,Homo sapiens,Canis lupus familiaris,Sus scrofa,Rattus norvegicus and Gallus gallus were 97.8%,92.0%,91.3%,91.3%,91.1%,88.1% and 73.3%,respectively.The physicochemical properties of ACSS2 protein showed that its molecular weight was 78.72 ku,and its theoretical isoelectric point was 6.03,which belonged to an acidic protein.No transmembrane structure and signal peptide was found in ACSS2 protein,but it contained a N-terminal domain of acetyl-CoA synthetase.Subcellular localization analysis indicated that ACSS2 protein was located in endoplasmic reticulum (44.4%),mitochondria (33.3%),cytoplasm (11.1%) and nucleus (11.1%).ACSS2 protein structure was mainly consisted of alpha helix (29.10%),extended chain (21.54%),beta turn(9.84%) and random coil (39.52%).Real-time PCR results revealed that the highest and the lowest expression of ACSS2 gene in goats were in the middle lactation and dry lactation periods,respectively.These results provided references for further study of the function and transcriptional regulation mechanism of ACSS2 gene in lipid metabolism of goats.

Key words: goat; ACSS2 gene; cloning; bioinformatics analysis; expression analysis

CLC Number:

SONG Guohua, YAN Duo, LI Ruiting, XU Huifen, LI Ming. A Cloning,Sequence Analysis and Tissue Expression of ACSS2 Gene in Goats[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 336-344.

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A Cloning,Sequence Analysis and Tissue Expression of ACSS2 Gene in Goats

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