单增李斯特菌溶血素S llsB基因缺失株的构建及部分生物学特性研究

doi:10.16431/j.cnki.1671-7236.2018.12.006

Abstract

Abstract:

In order to analyze the effect of listeriolysin S (LLS) llsB gene deletion on the biological characteristics of Listeria monocytogenes (LM),this study used homologous recombination to construct the llsB gene deletion strain LM90-ΔllsB,and the biological characteristics of growth characteristics,median lethal dose (LD₅₀) and organ-borne bacteria were studied in healthy Kunming male mice at 8 weeks old and weighing 40 g±5 g.The llsB gene deletion strain was successfully constructed,and the deletion strain had good genetic stability through continuous passage to 20 generations in vitro.Based on its growth curve examination,we found that the growth rate of the mutant strain was slightly higher than that of the parent strain.The results of mice infection test showed that the LD₅₀ of the parent strain and the deletion strain were 10^6.17 and 10^6.50 CFU,respectively.Compared with the parent strain,the amount of bacteria load of the deletion strain in the liver and spleen of the mice was extremely significantly decreased (P<0.01),the results showed that the infection ability of the mutant strain on mice was obviously weakened.No Listeria monocytogenes was detected in the brain.The results suggested that llsB gene might have direct or indirect regulatory effect on some biological characteristics of LM90,and it would provide a theoretical basis for further understanding of the pathogenic mechanism of LLS,prevention and control of listeriosis.

Key words: listeriolysin S (LLS); llsB gene; gene mutant; biological characteristics

CLC Number:

S852.61

YIN Hua, REN Jingjing, WANG Xinyu, JIANG Jianjun. Construction and Partial Biological Characteristics Analysis of Listeriolysin S llsB Gene Deletion Strain[J]. , 2018, 45(12): 3355-3362.

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Construction and Partial Biological Characteristics Analysis of Listeriolysin S llsB Gene Deletion Strain

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