乌蒙凤鸡生长激素基因多态性及生物信息分析

doi:10.16431/j.cnki.1671-7236.2018.11.018

Abstract

Abstract:

This study was aimed to explore and analyze the polymorphisms of growth hormone (GH) gene in Wumeng crested chickens.Taking Wumeng crested chickens as the research materials,the blood genomic DNA was extracted to construct DNA pool,all exons and partial introns of GH gene were amplified by PCR,the polymorphisms of GH gene was detected by sequencing,the secondary structure,tertiary structure and basic properties (physicochemical properties,hydrophobicity,signal peptide,transmembrane region,coiled-coil region and conserved domain) of GH protein were analyzed using bioinformatics softwares.The results showed that there were 8 SNPs(T1453C,A1489G,A1512G,G2152A,G3206A,G3347A,C3452A and C3453G) of GH gene in Wumeng crested chickens,there were 3 SNPs(G2152A,C3452A and C3453G) only appeared in flathead Wumeng crested chickens.The polymorphic sites were in different regions,C3452A and C3453G were located in the non-coding region,T1453C,A1489G,A1512G,G2152A and G3206A were located in introns region,and G3347A was located in exon 5.After mutation,the mRNA structure of GH gene were changed,which made the minimum free energy increases,and the stability decreases.Bioinformatics analysis result indicated that GH protein was secreted proteins,which had signal peptide sequence,a typical helical coil structure and a hydrophobic region,there was also a conserved domains in 10-214 amino acids.However,it was not a trans-membrane protein,and the stability was low.The results suggested that GH gene in Wumeng crested chickens had high genetic diversity,which could provide a reference for the conservation breeding of Wumeng crested chickens.

Key words: Wumeng crested chickens; crested; GH gene; polymorphism; bioinformatics

CLC Number:

S813.3

LU Jing, LUO Keyin, WANG Xianke, ZHANG Xiong, YANG Hong, MO Xianting, HUANG Mingjie, ZHANG Yong. Polymorphism and Bioinformatics Analysis of Growth Hormone Gene in Wumeng Crested Chickens[J]. , 2018, 45(11): 3122-3133.

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Polymorphism and Bioinformatics Analysis of Growth Hormone Gene in Wumeng Crested Chickens

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