乙酰甲喹在斑马鱼中的代谢研究

doi:10.16431/j.cnki.1671-7236.2018.10.031

Abstract

Abstract:

This study was aimed to illustrate the metabolic pathway of mequindox (MEQ) in aquatic animal.Ultra-high performance liquid chromatography tandem time-of-flight mass spectrum (UPLC-Q/TOF-MS) and metabolism analysis software Metabolynx^XS were adopted in this research,and zebrafish was adopted as aquatic model animal for MEQ metabolism investigation.After dipping with MEQ,zebrafish tissues were collected and MEQ metabolites were extracted with ethyl acetate and acetonitrile in turn.Then samples were filter through 0.22 μm filter membrane for further UPLC-Q/TOF-MS analysis.The number of metabolites could be confirmed according to the newly increased chromatographic peak by comparing the experimental group and the control.The chemical structure of metabolites could be confirmed according to the accurate mass spectrum by comparing the metabolites and the MEQ standard.The results showed that the major metabolic pathway of MEQ in zebrafish was N→O group reduction.Only three metabolites were identified,including N1-deoxymequindox (1-DMEQ),N4-deoxymequindox (4-DMEQ) and N1,N4-bisdeoxymequindox (1,4-BDMEQ).The depletion rule of MEQ and major metabolites in zebrafish was investigated.It was found that the concentration of MEQ reduced after administration,and it was less than the half of the highest concentration after 4 h.As to the three metabolites,the concentration first increased and then decreased.The highest concentration of 1-DMEQ and 4-DMEQ was at 2 h after administration,and then it was less than the half of the highest concentration after 8 h.For another metabolite 1,4-BDMEQ,the highest concentration was at 4 h after administration,and it was less than the half of the highest concentration after 12 h.This study would contribute to further control of aquatic animal derived food safety.In addition,the results would be benefit for the metabolism and pharmacokinetic investigation of MEQ in other aquatic food animal.

Key words: mequindox; metabolism; zebrafish; aquatic food animal

CLC Number:

S948

MAO Xin, TAN Haiguang, CHU Yang, LIU Huihui, YU Aijie, WANG Yunhui, LI Yanshen. Metabolism Investigation of Mequindox in Zebrafish[J]. , 2018, 45(10): 2927-2935.

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Metabolism Investigation of Mequindox in Zebrafish

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