黔北麻羊MEF2D基因多态性及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.10.002

Abstract

Abstract:

This study was aimed to screen the polymorphism of myocyte enhancer factor 2D(MEF2D) gene in Qiabbei Ma goat,and analyze the genetic structure by bioinformatics method.Primers were designed according to Capra hircus MEF2D gene sequence in GenBank (accession No.:NC_030810),the mixed DNA pool and PCR products combined with direct sequencing were used to obtain the polymorphisms of exons 2,3,4,5 and 6 of MEF2D gene,the phylogenetic tree of MEF2D gene,physicochemical properties,hydrophobicity,transmembrane structure,signal peptide and secondary structure of MEF2D protein in Qianbei Ma goat were analyzed using bioinformatics analysis software.The results showed that three SNPs were screened,namely Exon3-G17617A,Exon5-C20180A and Exon5-C20259T.Phylogenetic tree analysis showed that the genetic relationship were most closed between Qianbei Ma goat and Bos taurus,and were more closely related to Sus scrofa and Homo sapiens than Rattus norvegicus and Mus musculus.Protein physical and chemical properties analysis showed that the theoretical isoelectric point of MEF2D protein was about 7.74,the minimum value of hydrophilicity was -2.867,the maximum value of hydrophobicity was 1.933,which was not a transmembrane protein and no signal peptide existed.The secondary structure showed that the increase of α-helix and extended chain,the decrease of β-turn and random coil of MEF2D protein Exon5-C20180A in Qianbei Ma goat.This result indicated that the polymorphism of MEF2D gene in Qiabbei Ma goat was successfully screened,which provided a foundation for the study of molecular genetic marker-assisted selection in breeding of Qianbei Ma goat.

Key words: Qianbei Ma goat; MEF2D gene; polymorphism; bioinformatics

CLC Number:

S813.3

LUO Zheng, WENG Jimei, FENG Junqi, XU Wei, HUANG Lan, LUO Weixing, CAI Huifen. Polymorphism and Bioinformatic Analysis of MEF2D Gene in Qianbei Ma Goat[J]. , 2018, 45(10): 2661-2669.

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Polymorphism and Bioinformatic Analysis of MEF2D Gene in Qianbei Ma Goat

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