L-茶氨酸对氧化应激断奶仔猪生长性能及血液生化指标的影响

doi:10.16431/j.cnki.1671-7236.2018.08.016

Abstract

Abstract:

To explore the effects of L-theanine on growth performance,antioxidant and immune response of oxidative stress weaned pigs,a two-factor (2×2) experimental design was employed.160 weaned pigs (7.53 kg±0.51 kg) were divided into 4 treatment groups:Treatment 1 (without L-theanine,intraperitoneal injection of 5 mL normal saline),treatment 2(without L-theanine,intraperitoneal injection of 8 mg/(kg·BW) diquat),treatment 3(diet added 1 000 mg/kg L-theanine,intraperitoneal injection of 5 mL normal saline) and treatment 4 (diet added 1 000 mg/kg L-theanine,intraperitoneal injection of 8 mg/(kg·BW) diquat).7 d for preliminary feeding and 28 d for formal feeding.The results showed that:① Compared to treatment 1,the average daily gain (ADG) and average daily feed intake (ADFI) of weaned pigs in treatment 2 decreased significantly (P<0.05),while F/G increased significantly (P<0.05);Compared to treatment 2,L-theanine significantly increased ADG and ADFI in treatment 4 (P<0.05).②Compared to treatment 1,diquat significantly increased serum malondialdehyde (MDA) content,while the total antioxidant capacity (T-AOC) and the activity of glutathione peroxidase (GSH-Px) significantly decreased in treatment 2 (P<0.05),serum MDA content in treatment 3 significantly increased while the activity of GSH-Px significantly tended down (P<0.05);Compared to treatment 2,L-theanine significantly decreased serum MDA content while increased the activity of T-AOC and GSH-Px in treatment 4 (P<0.05).③Compared to treatment 1,the levels of serum IgA,IgG and IL-2,IL-4 significantly increased,while C3 and C4 significantly decreased (P<0.05) in treatment 2,and the levels of serum IgA,IgG and IL-4 were significantly decreased,while the concentration of C3 and C4 significantly increased in treatment 3 (P<0.05);Compared to treatment 2,the levels of serum IgA,IgG and IL-2,IL-4 significantly decreased,while C3 significantly increased in treatment 4 (P<0.05).In conclusion,injection of diquat could cause oxidative stress in piglets,resulting decrease of growth performance and humoral immunity;Diets added 1 000 mg/kg L-theanine could relieve oxidative damage,improve the growth performance,the capacity of antioxidation and humoral immunity in oxidative stressed piglets.

Key words: L-theanine; weaned piglet; oxidative stress; growth performance; humoral immunity

CLC Number:

S816.7

YAN Jun, LI Zeqing, WANG Wenjie, LIU Tong, MA Yanxuan, LI Ning, MA Yong, ZHENG Zi, MU Shuqin. Effects of L-theanine on Growth Performance and Blood Biochemical Indicators of Oxidative Stress Weaned Piglets[J]. , 2018, 45(8): 2175-2181.

References

[1] 徐静.猪氧化应激模型构建以及茶多酚的抗应激效应的研究[D].雅安:四川农业大学,2009.XU J.Study on the model of oxidative stress and antioxidative effects of tea polyphenols in pigs[D].Ya'an:Sichuan Agricultural University,2009.(in Chinese)
[2] UNNO K,TANIDA N,ISHⅡ N,et al.Anti-stress effect of theanine on students during pharmacy practice:Positive correlation among salivary α-amylase activity,trait anxiety and subjective stress[J].Pharmacology Biochemistry & Behavior,2013,111:128-135.
[3] NAGAI K,ODA A,KONNISHI H.Theanine prevents doxorubicin-induced acute hepatotoxicity by reducing intrinsic apoptotic response[J].Food & Chemical Toxicology,2015,78:147-152.
[4] CHATTERJEE S,CHATTERJEE A,ROY S,et al.L-theanine healed NSAID-induced gastric ulcer by modulating pro-antioxidant balance in gastric ulcer margin[J].Journal of Natural Medicines,2014,68(4):699-708.
[5] 刘昆言,丰金玉,肖文军.茶氨酸合成及生理作用研究进展[J].茶叶通讯,2014,4:3-7.LIU K Y,FENG J Y,XIAO W J.Research progress on biosynthesis and physiological function of thean-ine[J].Journal of Tea Communication,2014,4:3-7.(in Chinese)
[6] 陈义勇,窦祥龙,黄友如,等.茶氨酸的超声-微波提取工艺及其抗氧化活性[J].食品工业,2014,35(10):165-167.CHEN Y Y,DOU X L,HUANG Y R,et al.Optimization of ultrasonic microwave assisted extraction technology of theanine from tea and its antioxidant activity[J].Food Industry,2014,35(10):165-167.(in Chinese)
[7] 陈淑珍,甄永苏.茶氨酸的药理作用及机制[J].医学研究杂志,2013,42(11):17-20.CHEN S Z,ZHEN Y S.Pharmacological action and mechanism of theanine[J].Journal of Medical Research,2013,42(11):17-20.(in Chinese)
[8] 李桂兰,抗晶晶,殷志敏.L-茶氨酸对H₂O₂致LO2细胞损伤的保护作用及其机制研究[J].中国细胞生物学报,2014,36(6):785-790.LI G L,KANG J J,YIN Z M.Protective effect and the mechanism of L-theanine H₂O₂ against induced injury in LO₂ cells[J].Journal of Chinese Cell Biology,2014,36(6):785-790.(in Chinese)
[9] HWANG Y H,PARK B K,LIM J H,et al.Effects of beta-glucan from Paenibacillus polymyxa and L-theanine on growth performance and immunomodulation in weanling piglets[J].Asian-Australasian Journal of Animal Sciences,2008,21(12):1753-1759.
[10] 文慧,魏时来,张石蕊,等.L-茶氨酸对黄羽肉鸡生产性能和免疫功能的影响[J].动物营养学报,2012,24(10):1946-1954.WEN H,WEI S L,ZHANG S R,et al.Effects of L-theanine on performance and immune function of Yellow-feathered broilers[J].Chinese Journal of Animal Nutrition,2012,24(10):1946-1954.(in Chinese)
[11] 汪丽伟,蔡海莹.茶多酚和L-茶氨酸对热应激下蛋用仔公鸡生长性能及抗氧化能力的影响[J].中国畜牧兽医,2013,40(12):76-80.WANG L W,CAI H Y.Effects of tea polyphenol and L-theanine on growth performance and antioxidant performance of egg-laying chickens under heat stress[J].Chinese Animal Husbandry & Veterinary Medicine,2013,40(12):76-80.(in Chinese)
[12] 李宁,闫峻,穆淑琴,等.日粮添加L-茶氨酸对育肥猪采食行为的影响[J].粮食与饲料工业,2016,12(10):52-54.LI N,YAN J,MU S Q,et al.Effects of L-theanine on intake behavior in finishing pigs[J].Food and Feed Industry,2016,12(10):52-54.(in Chinese)
[13] 彭彬,刘仲华,林勇,等.L-茶氨酸改善慢性应激大鼠抑郁行为作用研究[J].茶叶科学,2014,4:355-363.PENG B,LIU Z H,LIN Y,et al.The ameliorative effect of L-theanine on chronic unpredictable mild stress-induced depression in rats[J].Journal of Tea Science,2014,4:355-363.(in Chinese)
[14] 刘晓慧,揭国良,林康,等.EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究[J].茶叶科学,2014,3:239-247.LIU X H,JIE G L,LIN K,et al.Research on synergistic protection and repairing effects of EGCG and theanine in oxidative damaged cells[J].Journal of Tea Science,2014,3:239-247.(in Chinese)
[15] KUEIHARA S,SHIBAHARA S,ARISSAKA H,et al.Enhancement of antigen-specific immunoglobulin G production in mice by co-administration of L-cystine and L-theanine[J].Journal of Veterinary Medical Science,2007,69(12):1263.
[16] 孙浪,鲁双庆,肖调义,等.饲料中添加茶氨酸对草鱼免疫和血清生化指标的影响[J].饲料研究,2012,6:63-65.SUN L,LU S Q,XIAO D Y,et al.Effects of adding tea amino acid on the immune and serum biochemical indexes of grass fish[J].Feed Research,2012,6:63-65.(in Chinese)
[17] JIANG T,ZHOU C,REN S.Role of IL-2 in cancer immunotherapy[J].Oncoimmunology,2016,5(6):e1163462.
[18] 谢哲,肖长纪,吴求吉,等.补体系统与细胞因子的相互作用[J].医学分子生物学杂志,2009,6(5):468-471.XIE Z,XIAO C J,WU Q J,et al.Complement system and cytokines[J].Journal of Molecular Biology of Medicine,2009,6(5):468-471.(in Chinese)
[19] KORKMAZ H I,ULRICH M M W,VAN WIERINGEN W N,et al.The local and systemic inflammatory response in a pig burn wound model with a pivotal role for complement[J].Journal of Burn Care & Research,2017,38(5):e796-e806.
[20] RAYES J,ING M,DELIGNAT S,et al.Complement C3 is a novel modulator of the anti-FⅧ immune response[J].Haematologica,2018,103(2):351-360.

Effects of L-theanine on Growth Performance and Blood Biochemical Indicators of Oxidative Stress Weaned Piglets

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Chen, GE Sheng, NING Xiaqing, WANG Jiaqi, ZHANG Nuannuan, WANG Xiaoran, ZHANG Shixia. Oxidative Stress Model of BRL-3A Cells Induced by Sodium Hydrosulfite [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2217-2223.
[2]	WANG Jing, SONG Hongbing, WU Yanling, LIN Weimin, XIAO Tianfang. Research Progress of Crocin in Animal Antioxidants [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2233-2244.
[3]	LI Rui, ZHANG Yiyang, LUO Xuehui, ZHOU Manlin, WU Shaomi, YANG Kun, QIAO Zilin. Effect of Hypoxia on Proliferation and Oxidative Stress Injury of Pulmonary Artery Smooth Muscle Cells in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2245-2254.
[4]	LI Yanghui, XU Tao, ZHANG Shuxian, LIU Ke, JIA Tianqing, CHEN Ning, YIN Junliang, XI Linqiao, WANG Dong, ZHOU Xiaoling. Effect of Replacing Wheat Straw with Cumin Straw on Growth Performance, Slaughter Performance and Plasma Biochemical Parameters of Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2296-2304.
[5]	WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.
[6]	LI Yongqiang, BAO Ruiying, WANG Qin, QIU Pingfei, LI Xiaochun, SHI Huiyu, ZHANG Haiwen, WANG Xuemei. Protective Effects of Antioxidant Peptides from Maggot on Oxidative Stress Injury of IPEC-J2 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1836-1844.
[7]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[8]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[9]	ZHANG Jinwu, WEI Zi, JI Yutong, WU Changxu, LI Zhenmin, WEI Yingyi, HU Tingjun, YU Meiling. Study on Oxidative Stress and Inflammatory Injury in Intestinal Tract of Yellow-feathered Chickens Induced by Clostridium perfringens Type A [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2069-2081.
[10]	REN Jiangang, GUAN Peng, HUANG Jianzhi, SHENG Yueyun, BAO Tiezhu, MA Zhiping, QIAN Wenxi. Effects of Dietary Energy Level on Growth-fattening Performance and Serum Biochemical Indices of Angus Bulls [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 966-976.
[11]	XIONG Taidi, JIANG Shouqun, ZHANG Huihua, ZHU Cui. Research Progress on Transportation Stress and Its Mitigation Measures in Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 977-988.
[12]	DOU Jiahong, WANG Ziying, YANG Juan, ZHOU Weiwei, ZHANG Tongcun, HE Hongpeng, DAI Xiaofeng, LI Xiumei. Analysis of the Active Ingredients and Mechanism of Radix Paeoniae Rubra Antioxidative Stress Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1229-1240.
[13]	LI Bin, SHI Zhengling, ZENG Ziyou, HUANG Yingyan, LI Lin, DU Zilai, JI Yifan, WANG Di, YU Jie, MEI Shaofeng. Effects of Chicory on Growth Performance,Slaughter Traits and Meat Quality of Mahuang Broilers with Different Gender [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 500-511.
[14]	CHEN Jin, XIE Jinghao, HAN Yingqian, YANG Yanbin, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Effects of Euphorbiae humifusae Aqueous Extract in IPEC-J2 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 704-712.
[15]	LIU Bo, LI Meilin, LIU Xu, LIANG Shuangying, MU Baolong, ZHAO Wenting, GE Yansong. Effects of Adipose-derived Stem Cell Conditioned Medium Alleviates Oxidative Stress Injury Induced by Sodium Taurocholate and Trypsin [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 798-806.