富氢生理盐水干预小型猪肝脏缺血再灌注合并肝脏部分切除损伤中内质网应激影响的研究

doi:10.16431/j.cnki.1671-7236.2018.06.034

Abstract

Abstract:

In order to study hydrogen-rich saline protects effects against liver ischemia-reperfusion injury and hepatectomy by inhibiting endoplasmic reticulum stress in miniature pig,18 3 to 4 months old and 20 to 30 kg body weight healthy miniature pigs were chosen and randomly divided into three groups:Sham group,model group and HRS group.Sham group only flipped the liver and maintained pneumoperitoneum for 3 h.Laparoscopic technique was employed to establish the model of hepatic ischemia-reperfusion combined with partial hepatectomy in model group.The surgical model was established in HRS group,and 10 mL/kg HRS was injected into the portal vein at 10 min before per-fusion and 1,2,3 d after surgery.Liver tissue samples were collected before operation,immediately after reperfusion,surgery,1 d after surgery and 3 d after surgery.HE staining and the mRNA expression of ERS-related parameters (PERK,IRE1,ATF6, GRP78,ATF4 and CHOP) in the liver were detected.The results showed that pathological changes of liver tissue in model group and HRS group were more serious than that in sham operation group,and ERS-related parameters mRNA level were increased in the model group and HRS group when compared with the sham group.In addition,the liver pathological damage in HRS group was significantly lighter than that in the model group,and all ERS-related parameters of mRNA levels were decreased in HRS group compared with the model group.Those results indicated that ischemia-reperfusion could induce liver ERS and injury,HRS could protect the liver from IRI by inhibiting ERS.

Key words: miniature pig; hydrogen-rich saline (HRS); hepatic ischemia-reperfusion; endoplasmic reticulum stress (ERS)

CLC Number:

S857.12

LI Hui, GE Yansong, BAI Ge, ZHANG Qianzhen, KONG Xiangdong, MENG Weijing, WANG Hongbin. Hydrogen-rich Saline Protects Effect Against Liver Ischemia-reperfusion Injury and Hepatectomy by Inhibiting Endoplasmic Reticulum Stress in Miniature Pig[J]. , 2018, 45(6): 1692-1699.

References

[1] BRAVO R, PARRA V,GATICA D,et al.Endoplasmic reticulum and the unfolded protein response:Dynamics and metabolic integration[J].International Review of Cell & Molecular Biology,2013,301:215-290.
[2] MOSBAH I B,ALFANY-FERNÁNDEZ I,MARTEL C,et al.Endoplasmic reticulum stress inhibition protects steatotic and non-steatotic livers in partial hepatectomy under ischemia-reperfusion[J].Cell Death & Disease,2010,1(7):e52.
[3] HETC C.The unfolded protein response:Controlling cell fate decisions under ER stress and beyond[J].Nature Reviews Molecular Cell Biology,2012,13(2):89-102.
[4] SAIDI R F,KENARI S K H.Liver ischemia/reperfusion injury:An overview[J].Journal of Investigative Surgery,2014,27(6):366-379.
[5] WEIGAND K,BROST S,STEINEBRUNNER N,et al.Ischemia/reperfusion injury in liver surgery and transplantation:Pathophysiology[J].HPB Surgery,2012,2012:176723.
[6] MALHI H, KAUFMAN R J.Endoplasmic reticulum stress in liver disease[J].Journal of Hepatology,2011,54(4):795-809.
[7] TABAS I,RON D.Integrating the mechanisms of apoptosis induced by endoplasmic reticulum stress[J].Nature Cell Biology,2011,13(3):184-190.
[8] CHANG W J,TOLEDO-PEREYRA L H.The potential benefits of hydrogen-rich saline in ischemia and reperfusion injury[J]. Journal of Surgical Research,2013,180(2):248-249.
[9] 张士霞,张建涛,范宏刚,等.腹腔镜技术在小动物中的应用[J].中国畜牧兽医,2012,39(12):172-174. ZHANG S X,ZHANG J T,FAN H G,et al.The application of laparoscopic technique in small animals[J].China Animal Husbandry & Veterinary Medicine,2012,39(12):172-174.(in Chinese)
[10] FUKUDA K I,ASOH S,ISHIKAWA M,et al.Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress[J].Biochemical & Biophysical Research Communications,2007,361(3):670-674.
[11] HAYASHIDA K,SANO M,OHSAWA I,et al.Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury[J].Biochemical & Biophysical Research Communications,2008,373(1):30-35.
[12] LI H,ZHOU R,LIU J,et al.Hydrogen-rich saline attenuates lung ischemia-reperfusion injury in rabbits[J].Journal of Surgical Research,2012,174(1):11-16.
[13] ZHENG X,ZHENG X,MAO Y,et al.Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats[J].Free Radical Research,2009,43(5):478-484.
[14] LEE J W,KIM J I,LEE Y A,et al.Inhaled hydrogen gas therapy for prevention of testicular ischemia/reperfusion injury in rats[J].Journal of Pediatric Surgery,2012,47(4):736-742.
[15] CHAUDHARI N,TALWAR P,PARIMISETTY A,et al.A molecular web:Endoplasmic reticulum stress,inflammation and oxidative stress[J].Frontiers in Cellular Neuroscience,2014,8:213-228.
[16] 周彦明,杨甲梅,殷正丰,等.内质网应激诱导的细胞凋亡与小鼠肝脏缺血再灌注损伤的关系[J].中国普通外科杂志,2009,18(8):870-872. ZHOU Y M,YANG J M,YIN Z F,et al.Relationship between endoplasmic reticulum stress induced apoptosis and liver ischemia reperfusion injury in mice[J].Chinese Journal of General Surgery,2009,18(8):870-872.(in Chinese)
[17] 石印青.内质网应激分子伴侣GRP78在大鼠肝脏I/R损伤中的表达及意义[D].西宁:青海大学,2014. SHI Y Q.endoplasmic reticulum stress molecular chaperone GRP78 in rat liver Ⅰ/R injury and its significance[D].Xining:Qinghai University,2014.(in Chinese)
[18] GAO Y,GUI Q,JIN L, et al.Hydrogen-rich saline attenuates hippocampus endoplasmic reticulum stress after cardiac arrest in rats[J].Neuroscience Letters,2017,640:29-36.
[19] IKUROH O,KEN-ICHIRO K,KIYOMI N,et al.Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals[J].Nature medicine,2007,13(6):688-694.
[20] LU Z,LIN Y,PENG B,et al.Hydrogen-rich saline ameliorates hepatic ischemia-reperfusion injury through regulation of endoplasmic reticulum stress and apoptosis[J].Digestive Diseases and Sciences,2017,62(12):3479-3486.
[21] FAN C,YANG Y,LIU Y, et al.Icariin displays anticancer activity against human esophageal cancer cells via regulating endoplasmic reticulum stress-mediated apoptotic signaling[J].Scientific Reports,2016,6:21145.
[22] RAO J,QIN J,QIAN X, et al.Lipopolysaccharide preconditioning protects hepatocytes from ischemia/reperfusion injury (IRI) through inhibiting ATF4-CHOP pathway in mice[J]. PLoS One,2013,8(6):e65568.
[23] KATAYAMA T,IMAIZUMI K,HONDA A,et al.Disturbed activation of endoplasmic reticulum stress transducers by familial Alzheimer's disease-linked presenilin-1 mutations[J].Journal of Biological Chemistry,2001,276(46):43446-43454.

Hydrogen-rich Saline Protects Effect Against Liver Ischemia-reperfusion Injury and Hepatectomy by Inhibiting Endoplasmic Reticulum Stress in Miniature Pig

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZOU Di-sha, YU Jian. Research Progress on Animal Models of Bama Miniature Pigs in the Field of Medicine [J]. , 2017, 44(4): 1128-1134.
[2]	FENG Shu-tang, MA Yu-yuan, XIA Ying. Research Progress and Countermeasures of Porcine Endogenous Retrovirus in Chinese Miniature [J]. , 2017, 44(4): 1244-1248.
[3]	LI Jun, XU Zhong-feng, ZHANG Hai-hang, SHANG Yue-li, KONG Xiang-feng, SHI De-shun, LIU Qing-you. Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs [J]. , 2016, 43(7): 1818-1825.
[4]	HUANG Jian-fang, LI Xiao-kai, JIANG Qin-yang, GUO Ya-fen, LAN Gan-qiu. Genetic Structure Analysis of Inbreeding Line in Guangxi Bama Miniature Pig [J]. , 2015, 42(2): 426-431.
[5]	HU Peng-fei，LI Xiang-chen，GUAN Wei-jun，MA Yue-hui. Prospects of Generation of Induced Pluripotent Stem Cell Lines from Wuzhishan Miniature Pig [J]. , 2014, 41(7): 174-177.
[6]	HUANG Yan-na, LAN Gan-qiu, JIANG Qin-yang. Research Progress on Molecular Biology of Guangxi Luchuan Pigs and Bama Miniature Pigs [J]. , 2014, 41(11): 79-84.
[7]	ZHANG He, WANG Cheng-li, WANG Yang, CHEN Ke-yan, SU Peng, SUN Qian. Research and Application of Miniature Pigs Models on Medical Domain [J]. , 2012, 39(7): 263-266.
[8]	LI Lian-feng, JIANG Qian, LIN Huan, LI Hong, LIU Jia-sen, GUO Dong-chun, YUAN Dong-wei, CUI Yu-dong, QU Lian-dong. Cloning and Sequence Analysis of the Main Cytokines in Bama Miniature Pig [J]. , 2012, 39(11): 56-61.
[9]	YAN Ke-song;ZHAN Da-wei;WANG Wei;AN Ning;WANG Jun-min. Hemostasis Effect of Disposable Packing Hemostasis on Miniature Pigs [J]. , 2010, 37(6): 239-241.
[10]	CHEN Wu;GU Jinni;JIANG Daixun;ZHANG Bing;LIU Jie;YU Tongquan;LU Ping;MU Xiang. Expression of Phosphodiesterase in Porcine Ovary and Influence of Relative Herbs on Its Activity [J]. , 2007, 34(6): 55-58.
[11]	YANG Shulin;REN Hongyan;WANG Heng;FENG Shutang;WANG Aide;GAN Shixiang;LI Kui. Investigation of the Blood Biochemistry Parameters of Three Chinese Indigenous Laboratory Miniature Pig Breeds [J]. , 2007, 34(4): 75-78.
[12]	YANG Shulin;REN Hongyan;WANG Heng;FENG Shutang;GAN Shixiang;WANG Aide;LI Kui. Investigation on the Hematology Parameters of Chinese Laboratory Miniature Pig Breeds [J]. , 2007, 34(2): 38-41.
[13]	JIN Erhui;SHAN Tongling;ZHANG Yong;MU Yulian;FENG Shutang;PENG Kemei;LI Kui;LIU Huazhen;TANG Wenhua;YANG Shulin . The Anatomic Study on the Digestive Organs in the Wuzhishan Miniature Pig Inbred-line [J]. , 2007, 34(12): 66-69.
[14]	JIN Erhui;PENG Kemei;ZHANG Yong;SHAN Tongling;MU Yunian;FENG Shutang;LI Kui;LIU Huazheng;TANG Wenhua;YANG Shulin . The Anatomic Study on the Heart of the Wuzhishan Miniature Pig Inbred-line [J]. , 2007, 34(11): 66-70.
[15]	YANG Shulin;JIN Erhui;SHAN Tongling;ZHANG Yong;MU Yulian;FENG Shutang;PENG Kemei;LIU Huazheng;TANG Wenhua;LI Kui. The Anatomic Study on the Urinary System of the Wuzhishan Miniature Pig Inbred-line [J]. , 2007, 34(10): 59-62.