表达猪圆环病毒2型衣壳蛋白的重组猪痘病毒的构建及鉴定

doi:10.16431/j.cnki.1671-7236.2018.02.001

Abstract

Abstract:

In order to investigate the feasibility of swinepox virus (SWPV) as porcine circovirus type 2 (PCV2) vaccine carrier, this study utilized promoter P11 of vaccinia virus launching the green protein screening markers, and used SWPV TK gene as the insertion of exogenous gene loci. P28 and P7.5 promoters started PCV2 ORF2 gene to construct two recombinant plasmids of rSWPV11-28C and rSWPV11-7.5C, respectively. SWPV JX20G strain was defined as the parent viruses, homologous recombination technology was used to build two strains (rSWPV11-28C and rSWPV11-7.5C), which expressed the PCV2 recombinant swinepox virus capsid protein. The results showed that the PCV2 capsid protein, which was successfully expressed in recombinant swinepox virus rSWPV11-28C and rSWPV11-7.5C, could react specifically with PCV2 monoclonal antibody 6E12. The PCV2 capsid protein promoted by the vaccinia virus promoter P28 was significantly higher than P7.5, P28 was suitable for starting the target gene. After immunization in mice via recombinant swinepox virus of PCV2 inoculated vaccine, the PCV2 of the rSWPV11-28C vaccine group and commodity vaccine showed equivalent antibody levels. The successful construction of the recombinant virus provided a new direction for the prevention and control of PCV2 related diseases and other diseases in swine.

Key words: porcine circovirus type 2(PCV2); capsid protein; recombinant swinepox virus

CLC Number:

S852.65

ZHONG Luohua, DENG Shunzhou, HU Yang, XU Changman, LUO Feng, ZHANG Wenbo, CHEN Songchang. Construction and Identification of Recombinant Swinepox Virus Expressing Porcine Circovirus Type 2 Capsid Protein[J]. , 2018, 45(2): 279-290.

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Construction and Identification of Recombinant Swinepox Virus Expressing Porcine Circovirus Type 2 Capsid Protein

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