猪圆环病毒2型ORF2基因重组植物乳杆菌的构建及其免疫原性分析

doi:10.16431/j.cnki.1671-7236.2017.08.027

Abstract

Abstract:

To develop an effective vaccine against porcine circovirus type 2 (PCV2), ORF2 gene was cloned into a Lactobacillus plantarum (L.plantarum) pSCPSP under the control of a SCP promoter to construct recombinant plasmid pSCPSP-Cap that was transformed into LP1 by electroporation. Extracellular expression of the Cap protein was confirmed by SDS-PAGE and Western blotting analysis. Afterwards, 8-week-old SPF BALB/c mice were randomly divided into control group, carrier group, lavage group, group of drinking water and inactived vaccine group. After 14,28,42 and 56 d, mice serums were collected to evaluate the level of specific antibody by SDS-PAGE and Western blotting. Eventually, SDS-PAGE and Western blotting results indicated that the Cap protein of PCV2 could be efficiently expressed in L. plantarum, expression of protein molecular weight was 28 ku and with good immunogenicity. In addition, higher levels of specific antibody in the serum of mice was observed by oral administration with L. plantarum expressing the PCV2 Cap antigen (P<0.05). In conclusion, the ORF2 gene of PCV2 could be successfully expressed in recombinant L. plantarum and expressed protein with good biological activity, which would lay the foundation for the development of PCV2 oral vaccination.

Key words: porcine circovirus type 2(PCV2); Cap protein; Lactobacillus plantarum; expression; oral vaccination

CLC Number:

S852.65

NI Neng-neng, WANG Xin-ke, LAI Qiang, JIAO Mao-xing, HUANG Yu-mao. Construction and Immunogenicity Analysis of Recombinant Lactobacillus plantarum Expressing ORF2 Gene of Porcine Circovirus Type 2[J]. , 2017, 44(8): 2408-2416.

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Construction and Immunogenicity Analysis of Recombinant Lactobacillus plantarum Expressing ORF2 Gene of Porcine Circovirus Type 2

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