银杏叶提取物对热应激致鸡心肌细胞氧化损伤的保护作用研究

doi:10.16431/j.cnki.1671-7236.2018.01.013

Abstract

Abstract:

The study was conducted to study the protective effect of Ginko biloba extract (GBE) on oxidative damage in cardiomyocytes of chicken induced by heat stress. The primary cardiomyocytes of 12-day-old AA broiler were prepared,0,5,10,50,100,150 mg/mL GBE were added and the cell vability was tested by CKK-8 method.And then the cardiomyocytes were inoculated and cultured for 48 h in 96-well plates,0,5,10,50,100,200,500 and 1 000 μg/mL GBE were added and the cell apoptosis were studied by Hoechst 33258 method to identify the proper concentration of GBE.The chicken cardiomyocyte heat stress model was established at 42℃ for 0,0.5,1,2 and 4 h,respectively. The LDH,GSH-Px,MDA and SOD were measured by ELISA method.Western blotting was used to detect the expression of Hsp72 protein.The results showed that high concentration of GBE was cytotoxic and 50,100,150 mg/mL GBE could extremely significantly inhibit cell growth (P<0.01),and cell proliferation rate was extremely significantly increased by 50 μg/mL GBE (P<0.01).Heat stress could induce cell apoptosis and oxidative damage through increasing the levels of MDA and LDH and reducing the GSH-Px level and SOD activity. GBE could reduce the levels of LDH and MDA and increase the SOD activity and GSH-Px level after heat stress,and the effect was more obvious when heat stress for 2 and 4 h. Furthermore,Western blotting results showed that GBE enhanced the expression of Hsp72.In conclusion,GBE could protect cardiomyocytes from oxidative damage induced by heat stress by increasing activities of antioxidative enzymes and Hsp72 expression.

Key words: Ginkgo biloba extract; cardiomyocytes; heat stress; oxidative damage

CLC Number:

S816.7

CHEN Hongbo, DUAN Dianning, WANG Hua. Protective Effects of Ginkgo biloba Extract on Oxidative Damage Induced by Heat Stress in Cardiomyocytes of Chicken[J]. , 2018, 45(1): 100-105.

References

[1] 鲍恩东,龚远英,付旭彬,等.肉鸡热应激病理损伤与应激蛋白(HSP70)相关性研究[J].中国农业科学,2004,37(2):301-305. BAO E D,HONG Y Y,FU X B,et al. Relation between pathologic damages and HSP70 changes in acute heat stressed broilers[J].Scientia Agricultura Sinica,2004,37(2):301-305. (in Chinese)
[2] 于纪棉.持续热应激肉鸡组织中热休克蛋白表达规律与应激性损伤机理研究[D].南京:南京农业大学,2009. YU J M. The mechanism of heat shock protein expressions and tissue damages in heat stressed broilers[D].Nanjing:Nanjing Agricultural University,2009.(in Chinese)
[3] DI W,MIAO Z,LU Y,et al. Aspirin-induced heat stress resistance in chicken myocardial cells can be suppressed by BAPTA-AM in vitro[J]. Cell Stress & Chaperones,2016,21(5):1-11.
[4] CHEN H B,ZHANG X C,CHENG Y F,et al. Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo[J]. Genetics & Molecular Research,2015,14:1994-2005.
[5] MOHAMED N E,El-MONEIM A E. Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats[J].Nutrition,2017,35:93-99.
[6] 阮海华,沈文飚,叶茂炳,等. 一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应[J].科学通报,2001,46(23):1993-1997. RUAN H H, SHEN W B,YE M B,et al. Protective effects of nitric oxide on oxidative damage in wheat leaves under salt stress[J]. Science Bulletin,2001,46(23):993-997. (in Chinese)
[7] 李毅,史小军,李新民,等. 槲皮素对过氧化氢所致PC-12细胞氧化损伤的保护作用[J].现代生物医学进展, 2012,12:6801-6804. LI Y,SHI X J,LI X M,et al. Protective effects of quercetin against hydrogen peroxide-induced oxidative damage in PC-12 cells[J]. Progress in Modern Biomedicine,2012,12:6801-6804. (in Chinese)
[8] 刘红艳,郭静明,王海燕,等.三氧化二砷对心肌细胞氧化损伤作用机制的实验研究[J].湖北中医药大学学报, 2012,14:11-12. LIU H Y,GUO J M,WANG H Y,et al. Effects of arsenic trioxide on myocardial cells from oxidative damage mechanism[J]. Journal of Hubei University of Chinese Medicine,2012,14:11-12. (in Chinese)
[9] 周孝琼,王华,陈慧芳,等.马齿苋不同提取物对肠炎沙门菌的体外抑菌试验[J].中国兽医杂志,2016,52:71-73. ZHOU X Q, WANG H, CHEN H F, et al. In vitro bacteriostatic test of different extracts from Portulaca oleracea on Salmonella enteritidis[J]. Chinese Journal of Veterinary Medicine,2016,52:71-73. (in Chinese)
[10] 刘志军.热应激心肌细胞内Hsp110表达有其抗热应激性损伤机理研究[D].南京:南京农业大学,2012. LIU Z J. The mechanism of Hsp110 expression in heat stress cardiomyocytes has its mechanism of heat stress injury[D].Nanjing:Nanjing Agricultural University,2012. (in Chinese)
[11] 吴向阳,仰榴青,陈钧.银杏茶中有毒成分银杏酸的研究[J].分析化学,2003,31:1407. WU X Y,YANG L Q,CHEN J. Study on a toxic component-ginkgolic acids in teas of Ginkgo biloba[J].Chinese Journal of Analytical Chemistry,2003,31:1407. (in Chinese)
[12] MUSTAFI S B,CHAKRABORTY P K,DEY R S,et al.Heat stress upregulates chaperone heat shock protein 70 and antioxidant manganese superoxide dismutase through reactive oxygen species (ROS),p38MAPK, and Akt[J]. Cell Stress & Chaperones,2009,14:579-589.
[13] KLUMPEN E,HOFFSCHROER N,ZEIS B,et al. Reactive oxygen species (ROS) and the heat stress response of Daphnia pulex:ROS-mediated activation of hypoxia-inducible factor 1(HIF-1) and heat shock factor 1(HSF-1) and the clustered expression of stress genes[J].Biology of the Cell,2017,109(1):39-64.
[14] SCHIEBER M,CHANDEL N S. ROS function in redox signaling and oxidative stress[J].Current Biology, 2014,24(10):453-462.
[15] ZOU P,XIA Y,CHEN T,et al. Selective killing of gastric cancer cells by a small molecule targeting ROS-mediated ER stress activation[J].Oncotarget,2016,7:1073-1086.
[16] 张美霞,叶志成. 银杏叶提取物对运动大鼠抗氧化效果的影响[J].食品研究与开发,2016,1:18-22. ZHANG M X,YE Z C. Effect of antioxidant on rats during exercise of Ginkgo biloba L. leaves extract(GBE)[J].Food Research and Developent,2016,1:18-22. (in Chinese)
[17] 梁翌,杨为民,李绪兰,等. 银杏叶提取物对兔血小板聚集的影响[J].中国药学杂志,2002,37(1):25-28. LIANG Y,YANG W M,LI X L,et al. Inhibitory effects of Ginkgo bibolia extract on platelet aggregation in rabbits[J].Chinese Pharmaceutical Journal,2002,37(1):25-28. (in Chinese)
[18] SCHNEIDER S M,ZUHL M N. HSP72 Up-regulation with heat acclimation[J]. Temperature Multidisciplinary Biomedical Journal,2016,3:28-30.
[19] GUPTA S,DEEPTI A,DEEGAN S,et al. HSP72 protects cells from ER stress-induced apoptosis via enhancement of IRE1α-XBP1 signaling through a physical interaction[J]. PLoS Biology, 2010,8:e1000410.
[20] 罗学滨,刘可,易宇欣,等.热休克蛋白72在氧化应激所致急性乳鼠培养心肌细胞损伤中的作用[J].中南大学学报(医学版),2006,31(2):228-231. LUO X B,LIU K,YI Y X,et al. Effect of HSP72 on acute injury of neonatal cardiomyocytes induced by oxidative stress[J]. Journal of Central South University (Medical Sciences),2006,31(2):228-231. (in Chinese)

Protective Effects of Ginkgo biloba Extract on Oxidative Damage Induced by Heat Stress in Cardiomyocytes of Chicken

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WU Yanan, SHI Yuxiang, ZHANG Yongying, LIU Guanhui, SONG Weiguang, ZHONG Cuihong, WANG Yongxia. Study on Nrf2 Activation Alleviating Heat Stress-Induced Oxidative Damage in Broiler Cardiomyocytes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3343-3352.
[2]	ZHANG Yuanxin, WANG Yanyu, YUN Yanhong, CHEN Junpu, SHI Huiyu, WANG Xuemei. Establishment of Oxidative Damage Model of Goat Mammary Epithelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1917-1925.
[3]	LIU Xinyong, TAN Zhengzhun, LI Hui, HUANG Jian, LUO Hua, ZENG Linghu, CHENG Juanru, ZHONG Huapei, YANG Chunyan, WANG Xiaoli, QIN Guangsheng. Effects of Heat Stress on Blood Biochemical Indexes of Murrah and Nili-Ravi Buffaloes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 521-528.
[4]	ZHU Gensheng, XIA Sugan, SHE Jinjin, BAI Yuni, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Protective Effects of Resveratrol on Zearalenone-induced Liver Oxidative Damage and Inflammation in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4254-4261.
[5]	TONG Xiong, MIN Li, SUN Hao, XU Bin, DING Diyun, LI Dagang, WANG Gang. Comparative Study on Physiological and Blood Biochemical Indexes of Leizhou, Nubia and Their F1 Crossbred Goats Under Heat Stress [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3625-3634.
[6]	CHEN Shaokan, LUO Hanpeng, ZHANG Guoxing, QIU Wenqing, DONG Yixin, WANG Yan, HUANG Xixia, WANG Yachun. Analysis of Differences in Hindgut Microbiota of Holstein Dairy Cows During Heat Stress [J]. , 2019, 46(8): 2273-2280.
[7]	HE Yuqiang, LI Yaokun, YANG Xinyue, LIU Guangbin, SUN Baoli, LIU Dewu. Research Advances on Heat Stress in Goats [J]. , 2018, 45(4): 1120-1126.
[8]	LIU Jiali, DOU Jinhuan, HU Lirong, XU Qing, WANG Yachun. Effects and Mechanism of Heat Stress on Physiological and Immune System in Dariy Cows [J]. , 2018, 45(1): 263-270.
[9]	HAN Jian-xin, HE Jian-bin, GAO Feng, YANG Shu-hua, LIANG Tian-tian, DONG Shuang, ZHANG Yi, LONG Miao. Protective Effects of Proanthocyanidins on the Liver and Kidney Oxidative Damage Induced by Zearalenone in Mice [J]. , 2016, 43(2): 402-406.
[10]	YE Wei-qing, DU Bing-wang, LI Dong-hua, LI Nai-bin. Establishment of Experimental Animal Models of Kirin Chicken (Chicken Feather Volume) Liver Injury Induced by Heat Stress [J]. , 2015, 42(3): 741-744.
[11]	ZANG Chang-jiang, ZHANG Jun-yu, WANG Lian-qun, GUO Tong-jun, BU Deng-pan, ZHOU Zhen-feng. Effects of Dietary Cation-anion Balance on Blood Biochemical Parameters of Heat Stressed Lactation Dairy Cows [J]. , 2014, 41(9): 95-100.
[12]	CHENG Jian-bo, WANG Wei-yu, ZHENG Nan, LI Song-li, ZHANG Yang-dong, ZHANG Xing-kai, YUAN Yao-ming, WANG Jia-qi. Natural Period Change of Heat Stress Reveals Unique “Heat-stressed Milk Protein Decrease Syndrome” in Mid-lactation Dairy Cows [J]. , 2014, 41(10): 73-84.
[13]	FU Chang-qi, XIA Jun-feng, LI Jin-lian, ZHOU De-fang, WANG Jian-cheng, SHI You-fei. Study on Effect of Taurochenodeoxycholic Acid on Heated-stressed Poultry and Mice [J]. , 2014, 41(10): 129-134.
[14]	YANG Feng, WANG Xu-rong, LI Xin-pu, LUO Jin-yin, WANG Ling, LI Hong-sheng. Beneficial Effect of N-acetylcysteine against Oxidative Damage in Mice Liver Induced by Streptococcus agalactiae Serotype Ⅰa Isolated from Cow [J]. , 2013, 40(8): 78-82.
[15]	FENG Yue-jin, GU Xian-hong. Research Progress on the Effect of Heat Stress on Meat Quality and its Mechanism in Pigs [J]. , 2013, 40(2): 96-99.