罗伊氏乳杆菌抑制致病性大肠杆菌感染效果的研究

doi:10.16431/j.cnki.1671-7236.2017.08.030

Abstract

Abstract:

This study was aimed to explore the effect,dose of Lactobacillus reuteri on inhibition of pathogenic Escherichia coli infection on Kunming mice as well as its potential mechanism. 96 Kunming mice were randomly allocated into four groups with 4 replicates per group and 6 mice per replicate,which were control group,low dose group,middle dose group and high dose group. All of the mice feed commercial basal feedstuff,meanwhile the mice in control group drank tap-water,three treatments were supplemented with 1.0×10⁶,1.0×10⁷ and 1.0×10⁸ CFU/mL Lactobacillus reuteri in water. The feeding period was 28 days. All the mice were weighted, and the ADFI,ADG and F/G were calculated at the end of experiment.At the day of 28,pathogenic Escherichia coli (4.98×10⁹ CFU/mL) were irrigated to study the mortality. The indexes of intestinotoxin and antioxidation in serum and cecal microbacteria were inspected.The results indicated that supplemented with middle dose of Lactobacillus reuteri could significantly improve the final body weight,ADG compared with the control (P<0.05). The mortality, serum intestinotoxin level and MDA concentration,Escherichia coli and Staphylococcus were significantly lower than control group (P<0.05),while the T-SOD,T-AOC,GSH-Px,Lacobacillus and Bifidobacterium were significantly improved (P<0.05).In conclusion,with Lactobacillus reuteri supplementation,the growth performance was improved and antioxidant capacity and cecal microbacteria were enhanced after pathogenic Escherichia coli infected, mouse mortality caused by the pathogenic Escherichia coli was effectively reduced,and the effect of 1.0×10⁷ CFU/mL Lactobacillus reuteri was the best.

Key words: Lactobacillus reuteri; Kunming mice; pathogenic Escherichia coli; antioxidant capacity

CLC Number:

S816.7

YANG Jia-jun, QIAN Kun, LI Qing-gang, WU Yi-jing, WU Dong, WANG Chong-long. Effects of Lactobacillus reuteri Supplementation on Inhibition of Pathogenic Escherichia coli in Kunming Mice[J]. , 2017, 44(8): 2431-2436.

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Effects of Lactobacillus reuteri Supplementation on Inhibition of Pathogenic Escherichia coli in Kunming Mice

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