不同海拔地区牦牛血液生化指标的比较研究

doi:10.16431/j.cnki.1671-7236.2018.11.022

Abstract

Abstract:

This study was designed to compare the myocardial enzymes activity,liver metabolism,kidney metabolism and some other biochemical indicators related to body antioxidant capacity,energy metabolism and lipid metabolism between yaks at two different altitudes.49 blood samples of yaks were collected in Datong,Qinghai (higher altitude) and 29 blood samples of yaks were collected in Hongsongwa farm,Hebei (lower altitude).25 blood biochemical indicators were tested and the results of the two groups were compared with independent t-tests.The results showed that,compared with yaks at lower altitude,the activities of blood ALT,AST,CK-MB,α-HBDH and LDH,the levels of blood TBiL,DBiL,TP,GLB,CRE,UA,MDA,and GLU in yaks at higher altitude were extremely significantly higher (P<0.01),and the activity of ALP were significantly higher (P<0.05) as well.While the SOD activity,NO,ALB and TG levels,and A/G were extremely significantly decreased (P<0.01).The rest of the indicators showed no significant difference (P>0.05).In conclusion,there were certain differences in myocardial enzyme activity,metabolic function and antioxidant capacity of yaks at two different altitudes,and yaks at higher altitude had been on the plateau for a long time and gradually developed the adaptability to the plateau hypoxia environment.

Key words: yak; myocardial enzymes activity; liver metabolism; kidney metabolism; antioxidant capacity; altitude acclimatization

CLC Number:

S823.8⁺5

LEI Lei, BAO Pengjia, WU Xiaoyun, CHU Min, LIANG Chunnian, YAN Ping. Comparison Study of Blood Biochemical Indicators of Yak at Different Altitudes[J]. , 2018, 45(11): 3160-3166.

References

[1] DING X Z,LIANG C N,GUO X,et al.Physiological insight into the high-altitude adaptations in domesticated yaks (Bos grunniens) along the Qinghai-Tibetan plateau altitudinal gradient[J] Livestock Science,2014,162(1):233-239.
[2] 胡琳,刘凤云,李愈娴,等.青海高原高山土生牦牛、高山迁饲黄牛与低海拔黄牛心肌酶谱测定分析[J].中国生化药物杂志,2015,35(6):29-30. HU L,LIU F Y,LI Y X,et al.Analysis on the myocardial enzyme spectrum of mountain yaks,alien mountain Yellow cattle and low altitude Yellow cattle in Qinghai plateau[J].Chinese Journal of Biochemical Pharmaceutics,2015,35(6):29-30.(in Chinese)
[3] 刘凤云,胡琳,李愈娴,等.慢性低氧时牦牛和迁饲黄牛血液肝酶指标的变化及其与ACE/ACE_2的相关性[J].中国应用生理学杂志,2015,31(3):272-275. LIU F Y,HU L,LI Y X,et al.Effect of altitude chronic hypoxia on liver enzymes and its correlation with ACE/ACE_2 in yak and migrated cattle[J].Chinese Journal of Applied Physiology,2015,31(3):272-275.(in Chinese)
[4] 李愈娴,刘凤云,胡琳,等.高原慢性低氧对土生牦牛与迁饲黄牛肾功能影响的比较[J].中国应用生理学杂志,2016,32(5):440-443. LI Y X,LIU F Y,HU L,et al.Comparative study of high altitude chronic hypoxia on renal function in yak and migrated cattle on Qinghai-Tibetan plateau[J].Chinese Journal of Applied Physiology,2016,32(5):440-443.(in Chinese)
[5] 李越,周月良,施佳慧,等.中国优秀女子赛艇运动员高原训练期间心肌酶谱等生理生化指标的变化[J].云南师范大学学报:自然科学版,2017,37(4):64-70. LI Y,ZHOU Y L,SHI J H,et al.Changes of biochemical and physiological and myocardium zymogram during altitude training in Chinese female rowing athletes[J].Journal of Yunan Normal University (Natural Sciences Edition),2017,37(4):64-70.(in Chinese)
[6] 葛雪微.高原环境人血清心肌酶与平原水平比较探讨[J].高原医学杂志,2009,19(4):53-55. GE X W.Comparison of serum myocardial enzymes of human beings at plateau and plain[J].Journal of High Altitude Medicine,2009,19(4):53-55.(in Chinese)
[7] 王延玲,王荣,杨锁泉,等.急进不同低海拔环境对大鼠心肌的影响[J].南方医科大学学报,2014,34(3):312-316. WANG Y L,WANG R,YANG S Q,et al.Myocardial injury in rats following a sudden increase of alti-tudes[J].Journal of Southern Medical University,2014,34(3):312-316.(in Chinese)
[8] 荣黎,曾维政,吴晓玲.高原缺氧与肝脏损伤[J].世界华人消化杂志,2009,17(21):2171-2178. RONG L,ZENG W Z,WU X L.High-altitude hypoxia and liver injury[J].World Chinese Journal of Digestology,2009,17(21):2171-2178.(in Chinese)
[9] LI W,JIA Z,XIE H,et al.Effects of acute exposure to high altitude on hepatic function and CYP1A2 and CYP3A4 activities in rats[J].Journal of Southern Medical University,2014,34(8):1203-1206.
[10] 万平新,万顺梅,杨丽红,等.高原低氧对大鼠肝脏氧化应激损伤的研究[J].胃肠病学和肝病学杂志,2016,25(1):84-86. WAN P X,WAN S M,YANG L H,et al.The research of hepatic oxidative stress injury in high altitude hypoxia in rats[J].Chinese Journal of Gastroenterology & Hepatology,2016,25(1):84-86.(in Chinese)
[11] PRATI D,TAIOLI E,ZANELLA A,et al.Updated definitions of healthy ranges for serum alanine aminotransferase levels[J].Annals of Internal Medicine,2002,137(1):1.
[12] 邓宇,高峰,周光宏.不同属种肉类对大鼠血清生化指标与抗氧化能力的影响[J].畜牧与兽医,2011,43(1):9-11. DENG Y,GAO F,ZHOU G H.Effects of different species meats on serum biochemical parameters and antioxidant status in rats[J].Animal Husbandry & Veterinary Medicine,2011,43(1):9-11.(in Chinese)
[13] BETRO M G,EDWARDS J B.Gamma-glutamyl transpeptidase in diseases of the liver and bone[J].American Journal of Clinical Pathology,1973,60(5):672-678.
[14] LUM G,GAMBINO S R.Serum gamma-glutamyl transpeptidase activity as an indicator of disease of liver,pancreas,or bone[J].Clinical Chemistry,1972,18(4):358-362.
[15] BARANANO D E,RAO M,FERRIS C D,et al.Biliverdin reductase:A major physiologic cytoprotectant[J].Proceedings of the National Academy of Sciences of the United States of America,2002,99(25):16093-16098.
[16] SEDLAK T W,SALEH M,HIGGINSON D S,et al.Bilirubin and glutathione have complementary antioxidant and cytoprotective roles[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(13):5171-5176.
[17] LIU Y,LI P,LU J,et al.Bilirubin possesses powerful immunomodulatory activity and suppresses experimental autoimmune encephalomyelitis[J].Journal of Immunology,2008,181(3):1887.
[18] 张亚萍,杨历新,宁梅桂,等.高海拔低氧对居民血清胆红素及相关指标的影响[J].陕西医学杂志,2003,32(8):705-707. ZHANG Y P,YANG L X,NING M G,et al.Effects of hypoxia on serum bilirubin and related indicators in residents at high altitude[J].Shanxi Medical Journal,2003,32(8):705-707.(in Chinese)
[19] APPELHOFF R J,TIAN Y M,RAVAL R R,et al.Differential function of the prolylhydroxylases PHD1,PHD2,and PHD3 in the regulation of hypoxia-inducible factor[J].Journal of Biological Chemistry,2004,279(37):38458-38465.
[20] BAE M K,AHN M Y,JEONG J W,et al.Jab1 interacts directly with HIF-1 alpha and regulates its stability[J].Journal of Biological Chemistry,2002,277(1):9.
[21] JIN H B,MAHON P C,OH J,et al.OS-9 interacts with hypoxia-inducible factor 1α and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1α[J].Molecular Cell,2005,17(4):503-512.
[22] 郭丹丹,董矜,郭晓东,等.不同海拔高原适应相关肝脏功能与脂代谢变化[J].现代生物医学进展,2013,13(1):71-74. GUO D D,DONG J,GUO X D,et al.Adaptive changes of liver function and lipid levels at different altitudes[J].Progress in Modern Biomedicine,2013,13(1):71-74.(in Chinese)
[23] 龙仁玲,何代平.拉萨地区藏汉族成人与儿童血清蛋白检测分析[J].西南军医,2007,9(1):50-51. LONG R L,HE D P.Analysis of serum proteins of adults and children of Tibet and Han nationality in Lhasa area[J].Journal of Military Surgron in Southwest China,2007,9(1):50-51.(in Chinese)
[24] 蒋世海,陈明华,杨平贵,等.不同海拔高度的白萨福克羊生理生化指标比较[J].黑龙江畜牧兽医,2015,11:106-108. JIANG S H,CHEN M H,YANG P G,et al.Comparison of the physiological and biochemical indexes of the White Suffolk sheep at different altitudes[J].Heilongjiang Animal Science and Veterinary Medicine,2015,11:106-108.(in Chinese)
[25] BARUA A,STANDEN N B,GALIÑANES M.Modulation of the nitric oxide metabolism overcomes the unresponsiveness of the diabetic human myocardium to protection against ischemic injury[J].Journal of Surgical Research,2011,171(2):452-456.
[26] 张鑫生,郭雪微,刘丽萍,等.不同海拔高度健康人超氧化物岐化酶和丙二醛的测定[J].中华医学杂志,1994,74(6):344-349. ZHANG X S,GUO X W,LIU L P,et al.Determination of superoxide dismutase and malondialdehyde in healthy people at different altitudes[J].National Medical Journal of China,1994,74(6):344-349.(in Chinese)
[27] 张西洲.青年人进驻不同海拔高度时血浆NO和NOS的变化[J].中国应用生理学杂志,2000,2:113. ZHANG X Z.Changes of plasma NO and NOS in young people entering different altitudes[J].Chinese Journal of Applied Physiology,2000,2:113.(in Chinese)
[28] HOLDEN J E,STONE C K,CLARK C M,et al.Enhanced cardiac metabolism of plasma glucose in high-altitude natives:Adaptation against chronic hypoxia[J].Journal of Applied Physiology,1995,79(1):222-228.
[29] 苏晓灵,周白丽,王嵘,等.高原不同海拔地区高脂血症患者血脂及血液流变学相关性研究[J].高原医学杂志,2009,19(4):8-11. SU X L,ZHOU B L,WANG R,et al.Related research of lipids and hemorheology in patients with hyperlipidemia at different geograpHic altitudes[J].Journal of High Altitude Medicine,2009,19(4):8-11.(in Chinese)

Comparison Study of Blood Biochemical Indicators of Yak at Different Altitudes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Rui, ZHANG Yiyang, LUO Xuehui, ZHOU Manlin, WU Shaomi, YANG Kun, QIAO Zilin. Effect of Hypoxia on Proliferation and Oxidative Stress Injury of Pulmonary Artery Smooth Muscle Cells in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2245-2254.
[2]	WU Zhijuan, CHAI Zhixin, WANG Jikun, WANG Jiabo, ZHONG Jincheng, XIN Jinwei. Expression Analysis of Specific Matrix Metalloproteinases and Functionally Related Genes of Skeletal Muscles in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1774-1784.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	WANG Yunlu, MENG Zhaoyi, YAO Yilong, GUO Min, NIU Jiaqaing, SUOLANG Sizhu, XU Yefen. Screening and Targeting Verification of lncRNA of "Sponge Adsorption" bta-miR-146a in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 859-869.
[5]	LEI Qi, REN Jie, KANG Jie. Effects of Scutellaria baicalensis georgi on Antioxidant Capacity,Sex Hormone Levels and mRNA Expression of ERα in Female Mice Exposed to ZEA [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 989-997.
[6]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[7]	ZHAO Luyuan, ZOU Chenhao, CAO Jiu'ai, GUO Yanshan, LIU Lijun, ZHAN Xiu'an, LIN Gang, LIU Bing, YU Dongyou. Effects of Low Level of Compound Organic Trace Minerals on Serum Biochemistry,Immune Function and Antioxidant Capacity of Meat Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 86-96.
[8]	WEI Jia, BAI Qin, LUO Xiaolin, GUAN Jiuqiang, AN Tianwu, ZHAO Hongwen, TAN Wu, LI Huade, XIE Rongqing, SHA Quan, JIANG Mingfeng, ZHANG Xiangfei. Effects of Different Weaning Strategies on Growth, Serum Biochemical Indexes and Antioxidant Capacity of Yak Calves [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3400-3410.
[9]	BAO Qi, BAO Pengjia, MA Xiaoming, WU Xiaoyun, MENG Guangyao, CHU Min, CAO Hongmei, LANG Jianying, AN Yufeng, LIANG Chunnian, YAN Ping. Genetic Structure,Selection Signal Analysis and ROH Detection of Sunan Yak Population [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2992-3005.
[10]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[11]	ZHANG Qiang, LUOSANG Dunzhu, BASANG Wangdui, PENG Yangyang, E Guangxin, NIMA Ciren, SUOLANG Duoji, BA Duo, DAN Ba, XIAN Lili, DANBA Jiacan, ZHI Zhang, PINGCUO Zhandui. Estimation of Genetic Diversity and Population Structure of Five Tibet Yak Populations Using Microsatellites Markers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2228-2238.
[12]	XIE Shuqiong, MA Shilong, TANG Jiao, LIU Yili, JIANG Mingfeng. Sequencing Analysis of Full-length Transcriptome of Abomasum in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1610-1620.
[13]	HAO Wenjun, XIA Hongze, JIAO Yang, CUI Zhanhong, LIU Shujie, ZHOU Lei, ZHOU Yuqing. Effects of Different Roughage Grading Index and Carbohydrate Balance Index Combinations Diets on Bacterial Flora in the Rumen and Rectum of Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1715-1726.
[14]	WU Yuan, QIN Xin, DONG Yuanyang, HAN Miaomiao, MIAO Zhiqiang, WEI Qingyu, SONG Xianyi, SHEN Liyan, FENG Yan, LI Huifeng, LI Jianghui. Effects of Dietary Methionine on Growth Performance, Antioxidant Capacity and Stress Tolerance of Broilers Under High-density Feeding [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 913-923.
[15]	ZHU Cui, HUANG Kaiyong, LI Lina, WU Aiji, WU Peng, FU Fangmiao, LUO Yuanhua, ZHANG Huihua. Effects of Berberine on Organ Index, Antioxidant Capacity and Intestinal Immunity in Yellow-feathered Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 932-941.