山羊CLAⅠα链基因的克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2017.07.023

Abstract

Abstract:

In order to explore the polymorphism of goat CLAⅠα chain gene, the primers were designed according to goat CLAⅠα chain gene in GenBank. Goat CLAⅠα chain gene was amplified from white blood cells of ten varieties of goats by RT-PCR,then the sequences were analyzed by bioinformatics. The results showed that the length of CLAⅠα chain gene was 1 074 bp, which encoded 357 amino acids. The nucleotide sequence analysis indicated that the homology of CLAⅠα chain genes of ten varieties of goats was 82.8% to 99.5%. The deduced amino acid sequence analysis revealed that the mutations were concentrated in α1 region (22-110 amino acids) and α2 region (111-203 amino acids). Genetic evolution analysis showed that the goat CLAⅠα chain gene in GenBank was close to the CLAⅠα chain gene of HSH-goat, HX-goat,SB-goat and AEBS-goat on genetic relationship, but with far relation to other 6 breeds, including JYDE-goat, JTH-goat, XNSN-goat, MG-goat, DZH-goat and NBY-goat. This study laid a theoretical foundation for the further study on polymorphism of CLAⅠα chain gene.

Key words: goat; CLAⅠα chain gene; cloning; bioinformatics analysis

CLC Number:

S852.65

DENG Yang, WU Guo-hua, YAN Xin-min, ZHAO Zhi-xun, LI Yang, LI Jian, DOU Yong-xi, ZHANG Zhi-dong, ZHANG Qiang. Cloning and Bioinformatic Analysis of Goats CLAⅠα Chain Gene[J]. , 2017, 44(7): 2065-2070.

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Cloning and Bioinformatic Analysis of Goats CLAⅠα Chain Gene

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