粤北地区新生仔猪腹泻病原学调查及PEDV ORF3基因分子特征和遗传变异分析

doi:10.16431/j.cnki.1671-7236.2017.06.027

Abstract

Abstract:

In order to understand the main pathogen of newborn piglets diarrhea in North Guangdong region, 31 diarrhea samples were collected from six pig farms in North Guangdong, the pathogen of porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PoRV) were detected by Real-time RT-PCR, meanwhile, ORF3 gene of PEDV amplified from positive samples were sequenced and analyzed. Pathogen detection results showed that 83.87%(26/31) samples were positive for PEDV, all herds and samples were negative for TGEV and PoRV. The sequence analysis revealed that the ORF3 gene of 5 epidemic strains of PEDV were all 675 bp, homologies of nucleotides were 98.7% to 100.0%, and homologies with reference sequences of nucleotides were 94.5% to 100.0%, and some gene mutation in common nucleotides site. The results of gene phylogenetic trees showed that PEDV could be divided into two groups, PEDV genetic relationship between field strains in North Guangdong and some regions in China, Southeast Asia, North America, Europe from 2013 to 2015 was closer, classed as a gene subgroup, from 2011 to 2012 main epidemic strains in our country and vaccine strains classed as other two gene subgroups. These results indicated that PEDV infection was the main pathogen of newborn piglets diarrhea in North Guangdong region, as time passed, the PEDV epidemic strains gene presented a tendency of evolution and variation.

Key words: diarrhea in newborn piglets; etiology; porcine epidemic diarrhea virus; ORF3 gene; genetic variation

CLC Number:

S858.28

NAN Wen-jin, HU Hong-hui, WU Jing-bo, HUANG Jian-qiang, PENG Guo-liang, XIAO Zheng-zhong. Etiological Investigation of Diarrhea in Newborn Piglets and Molecular Characterization and Genetic Variation Analysis of ORF3 Gene of PEDV in North Guangdong[J]. , 2017, 44(6): 1769-1777.

References

[1] 赵梦娇,陈书民,成岩,等. 山东省部分地区猪流行性腹泻流行病学调查及其M基因遗传进变异分析[J]. 中国兽医学报,2013,33(10):1504-1508.
[2] 王栓群,孙瑞芹,刘燕玲,等. 华南地区猪流行性腹泻病毒的分子流行病学调查与分析[J]. 广东农业科学,2015,42(6):97-104.
[3] Sun R Q,Cai R J,Chen Y Q,et al. Outbreak of porcine epidemic diarrhea in sucking piglets, China[J]. Emerging Infectious Diseases, 2012, 18(1):151-163.
[4] Chen J, Wang C, Shi H, et al. Complete genome sequence of a Chinese virulent porcine epidemic diarrhea virus strain[J].Journal of Virology, 2011, 85(21):11538-11539.
[5] 高继业,黄伟,王振业,等. 四川部分地区新生仔猪病毒性腹泻的病原学诊断[J].中国兽医杂志,2014,50(1):9-13.
[6] Debouck P, Pensart M. Experimental infection of pigs with a new porcine enteric coronavirus CV777[J].Am J Vet Res,1980,41(2):219-223.
[7] Chen J F, Sun D B, Wang C B, et al. Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China[J].Virus Genes,2008,36(2): 355-364.
[8] Wang K,Lu W, Chen J, et al. PEDV ORF3 encodes an ion channel protein and regulates virus production[J].FEBS Letters,2012,586(4):384-391.
[9] Pork S J, Moon H J, Luo Y, et al. Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea viruses[J]. Virus Genes, 2008, 36(1):95-104.
[10] Chen J, Wang C, Shi H, et al. Molecular epidemiology of porcine epidemic diarrhea virus in China[J].Archives of Virology,2010,155(9):1471-1476.
[11] 郑逢梅,霍金耀,赵军,等.2010-2012年华中地区猪流行性腹泻病毒分子特征和遗传进化分析[J].病毒学报,2013,29(2):197-205.
[12] 常铁城,陈建飞,冯力,等.2014年部分地区猪流行性腹泻病毒流行病学调查[J].中国预防兽医学报,2016,38(4):335-338.
[13] 冯旭芳,周碧君,张双翔,等.贵州省猪流行性腹泻病毒ORF3、M基因克隆及序列分析[J].中国畜牧兽医,2016,43(2):365-362.
[14] 卢冰霞,秦毅斌,何颖,等.2011年—2014年广西猪流行性腹泻病毒检测及其M基因的序列分析[J].中国畜牧兽医,2015,42(3):549-557.
[15] 王广操,陆倩倩,张梦岩,等. 2014—2015年我国华东部分地区猪流行性腹泻病毒的检测及S基因的序列分析[J]. 中国兽医科学,2016,46(6):702-709.
[16] 孙贤刚,唐蕊涵,蔡雨函,等. 2011—2012年四川省猪流行性腹泻病毒的遗传变异分析[J].中国兽医学报,2015,35(1):702-709.
[17] 张志,程小娜,樊雅婷,等.猪流行性腹泻病毒变异株的鉴定和分子特征分析[J].中国畜牧兽医,2015, 42(7):1654-1660.
[18] 常铁城.2014年中国11省市猪流行性腹泻病毒ORF3基因分子流行病学调查[D].大庆:黑龙江八一农垦大学,2015.
[19] 董彦鹏.2011—2012年我国猪流行性腹泻病毒ORF3基因分子流行病学调查与仔猪致病性研究[D].南京:南京农业大学,2012.
[20] Bae J L,Lee J G,Kang T J,et,al. Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing[J].Vaccine,2003,21(25):4052-4058.
[21] Song D S, Oh D S, Kang B K, et al. Oral efficacy of Vero cell attenuated porcine epidemic diarrhea viurs DR13 strain[J].Research in Veterinary Science,2007,82(1):134-140.

Etiological Investigation of Diarrhea in Newborn Piglets and Molecular Characterization and Genetic Variation Analysis of ORF3 Gene of PEDV in North Guangdong

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[2]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[3]	HAN Dayong, ZHOU Chunbao, NI Ligang, CHEN Zhangyan, ZHAO Jinming. Genetic Diversity of mtDNA D-loop Sequence in Sujiang Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1472-1479.
[4]	HUO Mingkai, GUAN Feihu, QIU Runhui, WEI Chunyan, YU Haitao, ZHU Jiale, ZHANG Hui. Study on the Mechanism of DDX1 Inhibiting Porcine Epidemic Diarrhea Virus Replication [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1556-1566.
[5]	LIU Jiaxing, HAN Xueying, ZHANG Xinru, DENG Jiaxin, YAO Lunguang, LIU Yangkun. Preparation and Immunogenicity Analysis of Ferritin Nanoparticles Carrying Antigenic Epitopes of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1567-1574.
[6]	WANG Shuaijie, TAN Zihan, LI Hanbo, SUN Xiangxue, AI Sihan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Quercetin on Intestinal Morphology, Antioxidant Function and Jejunal Lipid Metabolism Gene Expression in Piglets with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3391-3399.
[7]	WANG Manli, TAN Zihan, WANG Shuaijie, LI Qian, ZENG Yitong, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3411-3418.
[8]	ZHAI Xinguo, ZHENG Peipei, SU Jinhui, LI Qingyang, JIAO Hejing, ZHANG Ruobing, LI Penghui. Genetic Variation and Recombination Analysis of Whole Genome of Porcine Epidemic Diarrhea Virus HB/HEBEU/2020 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3140-3150.
[9]	SONG Shiying, GUO Weilu, XIA Xuefeng, ZHANG Xue, BI Zhenwei, ZHANG Xuehan, FAN Baochao, DONG Hailong, LI Bin. Expression and Purification of Porcine IFN-δ5 and Its Effect Analysis Against PEDV Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3171-3179.
[10]	LIU Yutong, YAO Yanting, LI Ruiling, HAN Wenqi, SHI Ting, WANG Yidan, REN Yupeng. Analysis of Genetic Variation of Porcine Epidemic Diarrhea Virus in Parts of Southwest China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2279-2290.
[11]	ZHANG Xinjie, XUE Shaohua, LYU Zixin, HUANG Xirong, CHEN Yuxuan, FAN Kewei, YANG Xiaoyan, DAI Ailing. Investigation of Co-infection of PPV7 and PCV2 and Genetic Variation Analysis for PPV7 Cap Gene in Fujian and Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2291-2297.
[12]	WANG Zihang, DENG Xingmei, QIU Runhui, ZHU Dexin, LI Jia, TAO Tingting, ZHU Jiale, SUN Zhihua, ZHANG Hui. Regulation of Long Noncoding RNAs in Autophagy Induced by PEDV Infected Vero-E6 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1942-1950.
[13]	ZHANG Mingliang, GENG Yachun, LIAN Kaiqi, MA Lei, CHANG Ying, WANG Shuangshan, ZHANG Fuliang. Cloning,Bioinformatics Analysis and Truncated Fragment Expression for Truncated M Gene of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1479-1487.
[14]	YAN Zhong, YANG Hanchun. Surveillance and Genetic Variation Analysis of S1 Gene of Porcine Epidemic Diarrhea Virus in Part Provinces and Municipalities of China from 2018 to 2020 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 424-434.
[15]	TAO Zhiyun, ZHU Chunhong, LIU Hongxiang, SHI Zuhao, ZHANG Shuangjie, XU Wenjuan, SONG Weitao, WANG Zhicheng, LI Huifang. Screening and Identification of Genetic Variation and Functional Genes Related to Egg Production in Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4107-4118.