猪卵母细胞在GV期与MⅡ期的冷冻保存效果比较研究

doi:10.16431/j.cnki.1671-7236.2017.03.026

Abstract

Abstract:

This study was designed to compare the efficiency of porcine oocytes vitrified at the GV and MⅡ stages. The vitrified GV oocytes were matured in vitro and then evaluated their cortical granule distribution with immunofluorescence, CD9 protein level with Western blotting and zona pellucida dissolution time with pronse digestion method, as compared to vitrified MⅡ oocytes.The results showed that the survival percentages of oocytes vitrified at the GV stage were significantly lower than those vitrified at the MⅡ stage after 2 h of warming (P<0.05);However, there was no difference in maturation rate between vitrified and fresh oocytes (P>0.05). The oocytes vitrified at MⅡ stage resulted in significantly decreased normal cortical granule distribution and CD9 protein level (P<0.05), but no such results were found in vitrified GV oocytes after maturation (P>0.05). In addition, vitrification did not affect the dissolution time of zona pellucida in GV and MⅡ oocytes (P>0.05). The data demonstrated that despite of lower survival rate compared with MⅡ oocytes, vitrified porcine GV oocytes after maturation showed normal polar body extrusion, cortical granule distribution and CD9 protein level.

Key words: porcine oocytes; vitrification; cortical granule; CD9; zona pellucida

CLC Number:

S814

WU Guo-quan, QUAN Guo-bo, LV Chun-rong, SHAO Qing-yong, HONG Qiong-hua. Comparative Effects of Porcine Oocytes Vitrified at the GV and MⅡ Stages[J]. , 2017, 44(3): 807-812.

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Comparative Effects of Porcine Oocytes Vitrified at the GV and MⅡ Stages

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