莱菔硫烷对染镉小鼠睾丸间质细胞抗氧化功能影响的研究

doi:10.16431/j.cnki.1671-7236.2017.02.037

Abstract

Abstract:

The study was aimed to explore the antioxident function of sulforaphane (SFN) on the leydig cells of cadmium (Cd) exposured mice.Cd and SFN were added to the cell culture medium of TM3 cell, half inhibitory concentration (IC₅₀) of Cd and SFN safe dose range were determined.The tested model was set up,and the relative survival rate of TM3 cells, lactate dehydrogenase (LDH) activity and cell antioxidant levels were determined to study the antioxident function of SFN to cadmium exposured mice.The results showed that:①With the increase of Cd concentration,the relative survival rate of TM3 cells was decreased,and the IC₅₀ of Cd was 51.4 μmol/L;Within a certain concentration range,SFN could increased the cells survive rate,but there was toxicity when the SFN concentration was more than the scope,and the greater the concentration,the greater the toxicity. At experimental condition,SFN safety concentration were 2.5,5 and 10 μmol/L.②Compared with the control group,the GSH content,the T-SOD and GSH-Px activities in Cd group were significantly or extremely significantly decreased (P < 0.05;P < 0.01),and the LDH activity,the MDA content were increased.Additionally,the LDH activity and MDA content of SFN groups were decreased,while others three indexes were increased. Compared with Cd group,the GSH content,the T-SOD and GSH-Px activities of Cd+SFN groups were significantly or extremely significantly increased (P < 0.05;P < 0.01),however,the LDH activity,MDA content were decreased. The result indicated that SFN had the antagonism effect on toxicity of Cd in TM3 cell.

Key words: cadmium; sulforaphane; TM3 cell; oxidative stress

CLC Number:

S858.91

JIANG Li-ying, HE Jian-bin, ZHENG Wei-ping, ZHANG Tong, LI Hui, YANG Shu-hua. Research on the Antioxident Function of Sulforaphane on the Leydig Cells of Cadmium Exposured Mice[J]. , 2017, 44(2): 568-577.

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Research on the Antioxident Function of Sulforaphane on the Leydig Cells of Cadmium Exposured Mice

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