鸭1型甲肝病毒亚型VP1蛋白单克隆抗体的研制及鉴定

doi:10.16431/j.cnki.1671-7236.2017.02.035

Abstract

Abstract:

To prepare the monoclonal antibodies (MAbs) against DHAV-1a, hybridomas were produced by fusing SP2/0 cells with spleen cells from mouse immunized with DHAV-1a pGEX-VP1 recombinant protein. Two hybridomas stablely secreting MAbs against VP1 protein were identified by indirect ELISA detection with DHAV-1a as coating antigen. The ascetic fluids of MAbs were 1:3.2×10⁴ and 1:2.0×10⁶, respectively. The MAbs were IgG1 with κ chain. Western blotting analysis showed that the MAbs could recognize the recombinant VP1 protein and DHAV-1a. The neutralization tests showed that one MAb (5G3) had better neutralization activity. Therefore, the results showed that the ELISA titers and specialities of two MAbs were very good, with excellent stability. In addition, they all had cross interaction with DHAV-1 and DHAV-1a, one of which had good neutralization activity.

Key words: DHAV-1a; monoclonal antibody; biological characteristics

CLC Number:

S852.65

FU Qiu-ling, LIU Wei, HUANG Yu, FU Guang-hua, WAN Chun-he, CHENG Long-fei, CHEN Hong-mei, SHI Shao-hua, LIU Rong-chang, CHEN Zhen, CHEN Cui-teng, LIN Jian-sheng. Development and Identification of Monoclonal Antibodies against VP1 Protein of DHAV-1a[J]. , 2017, 44(2): 554-560.

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Development and Identification of Monoclonal Antibodies against VP1 Protein of DHAV-1a

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