猪巨细胞病毒福建株DPOL基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2017.02.009

Abstract

Abstract:

In order to know the DPOL gene characterization of porcine cytomegalovirus (PCMV) (PCMV-FJ01 strain),three pairs of specific primer were designed by Oligo 7.0 software based on comparison the characterization of DPOL gene retrieved from GenBank.The target DPOL gene fragments were amplified using PCR methods with the strain PCMV-FJ01 genomic DNA. The target PCR fragments were cloned and sequenced. The assembly sequences were bioinformatics analysis. The DPOL gene of PCMV-FJ01 strain was 3 021 bp in length, coding 1 006 amino acids. The results showed that the homology of the nucleotide sequence and amino acid sequence were above 98.7% and 99.1%, respectively. Phylogenetic analysis revealed that the PCMV was closer with genus Roseolovirus rather than genus Cytomegalovirus. The results suggested that PCMV should be new species under genus Roseolovirus base on the phylogenetic relationship.

Key words: porcine cytomegalovirus; DPOL gene; clone; sequences analysis; virus classification

CLC Number:

S852.65⁺9.1

CHEN Ru-jing, CHEN Chen, WU Xue-min, CHE Yong-liang, YAN Shan, WANG Chen-yan, WANG Long-bai, ZHOU Lun-jiang. Cloning and Sequencing Analysis of DPOL Gene of Porcine Cytomegalovirus[J]. , 2017, 44(2): 365-370.

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Cloning and Sequencing Analysis of DPOL Gene of Porcine Cytomegalovirus

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