蓝舌病1型病毒VP7蛋白表(拟)位定位及免疫检测分析

doi:10.16431/j.cnki.1671-7236.2017.01.033

Abstract

Abstract:

In this study,in order to map the key amino epitope of VP7 protein of blue tongue virus serotype 1 (BTV1),we used monoclonal antibody against VP7 protein of BTV1 to screen 7 mer phage display random peptide library. The selected phages including VP7 protein consensus sequence were amplified and purified,immunoreactivity between epitope of selected phages and monoclonal antibody against VP7 of BTV1 was analyzed by ELISA and competitive ELISA (c-ELISA).The result showed that the epitope of phages including LNWPMVR sequence could specially combine monoclonal antibody against VP7 of BTV1,and the combination could be inhibited or blocked by BTV1,it demonstrated that 7 mer phage simulated the antigenic determinant of BTV protein which could combine monoclonal antibody against VP7 of BTV1.The results suggested that the amino acides 163 to 189(LNAGARGDVQQIFQGRNDPMMLYLVWR) were the specific epitope of VP7 protein of BTV.

Key words: bluetongue virus; VP7 protein; epitope

CLC Number:

S852.65

XIONG He-li, LI Guo-hua, WANG Jin-ping, MIAO Hai-sheng, SONG Jian-ling, LI Hua-chun. Epitope Mapping and Immuno-detection of VP7 Protein of Bluetongue Virus Serotype 1[J]. , 2017, 44(1): 236-241.

References

[1] Perez de Diego A C, Athmaram T N, Stewart M, et al. Pharacterization of protection afforded by a bivalent virus-like particle vaccine against bluetongue virus serotypes 1 and 4 in sheep[J].PLoS One,2011,6(10):e26666.
[2] 张念祖,李志华,张开礼,等. 中国蓝舌病发现历史、病毒分离及血清学研究[A]. 第一届东南亚太平洋地区蓝舌病毒学术研讨会论文汇编[C].昆明:1995:4-9.
[3] Matsuo E, Roy P. Minimum requirements for bluetongue virus primary replication in vivo[J].J Virol,2013,87(2):882-889.
[4] Belhouchet M, Mohd Jaafar F, Firth A E,et al. Detection of a fourth orbivirus non-structural protein[J].PLoS One,2011, 6(10):e25697.
[5] Yu Y, Fukusho A, Ritter D G, et al. Complete nucleotide sequence of the group-reactive antigen VP7 gene of bluetongue virus[J].Nucleic Acids Res, 1988, 16(4):1620.
[6] Hosamani M, Shimizu S, Hirota J, et al. Expression and characterization of bluetongue virus serotype 21 VP7 antigen:C-terminal truncated protein has significantly reduced antigenicity[J].J Vet Med Sci, 2011, 73(5):609-613.
[7] 熊和丽, 宋建领, 王金萍, 等. 应用噬菌体展示7肽文库技术进行蓝舌病1型病毒VP2蛋白表(拟)位分析[J]. 中国预防兽医学报,2015,37(10):786-789.
[8] Hofmann M A, Renzullo S, Mader M,et al. Genetic characterization of toggenburg orbivirus, a new bluetongue virus, from goats, Switzerland[J]. Emerg Infect Dis,2008,14(12):1855-1861.
[9] Maan S, Maan N S, Nomikouet K, et al. Novel bluetongue virus serotype from Kuwait[J].Emerg Infect Dis,2011,17(5):886-889.
[10] Jenckel M, Breard E, Schulz C, et al. Complete coding genome sequence of putative novel bluetongue virus serotype 27[J].Genome Announcements,2015, 3(2):e00016-15.
[11] Maan S, Maan N, Nomikou K, et al. Complete genome characterization of a novel 26th bluetongue virus serotype from Kuwait[J].PLoS One,2011, 6(10):e26147.
[12] Coetzee P, Stokstad M, venter E H, et al. Bluetongue:A historical and epidemiological perspective with the emphasis on South Africa[J].Virol J,2012, 9:198.
[13] Huismans H, Erasmus B J. Identification of the serotypespecific and group specific antigens of bluetongue virus[J].Onderstepoort J Vet Res,1983,48(2):51-58.
[14] Grimes J, Basak A K, Roy P, et al. The crystal structure of bluetongue virus VP7[J].Nature, 1995, 373(6510):167-170.
[15] 魏天, 徐青元, 耿宏伟, 等. 蓝舌病病毒VP7蛋白竞争抑制群特异性构象抗原表位的初步鉴定[J]. 中国预防兽医学报,2014,36(1):63-66.
[16] 耿宏伟, 秦永丽, 李俊平, 等. 蓝舌病病毒重组VP7蛋白单克隆抗体制备及竞争ELISA检测方法的建立[J]. 中国预防兽医学报,2012,34(5):388-392.
[17] 杜杰, 尹慧琼, 杨姝, 等.蓝舌病病毒VP7蛋白的B细胞表位预测[J]. 中国动物传染病学报,2013,21(1):29-36.
[18] 宋建领,张文东,张富强,等. 禽流感病毒M1蛋白型特异性表位分析[J]. 中国人兽共患病学报,2008,24(7):626-630.
[19] 张富强, 李志华, 张念祖. CSFV囊膜蛋白E2单克隆抗体识别表(拟)位基序的差异性分析[J]. 中国预防兽医学报, 2005, 27(5):373-379.

Epitope Mapping and Immuno-detection of VP7 Protein of Bluetongue Virus Serotype 1

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