大肠杆菌O157:H7 Ivy蛋白表位多肽及免疫原性分析

doi:10.16431/j.cnki.1671-7236.2017.01.027

Abstract

Abstract:

To evaluate the feasibility of synthetic polypeptides O157:H7 Ivy146-157 as a vaccine antigen, a bioinformatics method was employed to analyze the secondary structures, hydrophilicity plot, flexibility plot, surface probability plot and antigenic index of the Ivy. A B-cell epitope peptide sequence was identified and synthesized. BALB/c mice were immunized with Ivy146-157, antibody titers and splenic lymphocytes proliferations in mice were tested. The results showed that the antibody titers were 1:6 400 after immunized mice three times. And the native Ivy protein of O157:H7 R14 strain was identified by polyclone antibody Ivy146-157. The MTT assay results indicated the splenic lyphocytes were increased after immunized polypeptides Ivy146-157, and had significant difference with the control group (P<0.05). These results indicated that polypeptides Ivy146-157 could induce a strong humoral and celluar immune respond, and it would provide a theory as a vaccine antigen for further researches.

Key words: EHEC O157:H7; Ivy; polypeptides

CLC Number:

S852.4^＋3

PAN Yan, LIN Min-ling, LI Jun, YANG Wei, CHEN Ze-xiang, XIE Yong-ping, PENG Hao, HU TING-jun. Antigen Epitope Peptides and Immunogenicity Analysis of E. coli O157: H7 Ivy Protein[J]. , 2017, 44(1): 194-200.

References

[1] 秦恩强,赵敏. 新型肠出血性大肠杆菌感染防治[J].传染病信息,2011,24(3):127-128.
[2] Levine M M. Escherichia coli that cause diarrhea:Enterotoxigenic, enteropathogenic, enteroinvansive, enterohemorhagic and enteroadherant[J]. J Infect Dis,1987,155(3):377-389.
[3] Guner Y S, Malhotra A,Ford H R,et al. Association of Escherichia coli O157:H7 with necrotizing enterocolitis in a full-term infant[J].Pediatr Surg Int, 2009,25(5):459-463.
[4] Sankaran K,Puckett B,Lee D S,et al. Variations in incidence of necrotizing enterocolitis in Canadian neonatal intensive care units[J]. Pediatr Gastroenterol Nutr,2004,39(4):366-372.
[5] Fukushima H,Hashizume T,Morita Y,et al. Clinical experiences in Sakai City hospital during the massive outbreak of enterohemorrhagic Escherichia coli O157 infections in Sakai City,1996[J]. Pediatr Int,1999, 41(2):213-217.
[6] 王红, 李秀桂, 唐振柱, 等. 2002~2004年广西食品中肠出血性大肠杆菌O157:H7的监测分析[J]. 广西预防医学, 2005,11(5):286-297.
[7] 谢宇舟,密克,李军,等. 猪源大肠杆菌O157:H7与O157的差异蛋白质组学分析[J]. 基因组学与应用生物学,2012,31(6):531-537.
[8] Proctor V A,Cunningham F E. The chemistry of lysozyme and its use as a food preservative and a pharmaceutical[J]. Crit Rev Food Sci Nutr,1998,26(4):359-395.
[9] Ellison R T 3rd,Giehl T J. Killing of gram-negative bacteria by lactoferrin and lysozyme[J]. Clin Invest,1991,88(4):1080-1091.
[10] Monchois V,Abergel C,Sturgis J. Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme[J]. Biol Chem,2001, 276(21):18437-18441.
[11] Derbise A,Pierre F,Merchez M,et al. Inheritance of the lysozyme inhibitor Ivy was an important evolutionary step by Yersinia pestis to avoid the host innate immune response[J]. J Infect Dis, 2013, 207(10):1535-1543.
[12] 李军,禤雄标,谢宇舟,等.广西畜禽大肠杆菌O157:H7流行病学调查[J].中国人兽共患病学报,2011,27(12):1151-1155.
[13] Ganz T,Weiss J. Antimicrobial peptides of phagocytes and epithelia[J]. Semin Hematol,1997,34(4):343-354.
[14] Vanderkelen L,Van Herreweghe J M,Vanoirbeek K G,et al. Identification of a bacterial inhibitor against g-type lysozyme[J]. Cell Mol Life Sci,2011,68(6):1053-1064.
[15] Deckers D,Vanlint D,Callewaert L,et al. Role of the lysozyme inhibitor Ivy in growth or survival of Escherichia coli and Pseudomonas aeruginosa bacteria in hen egg white and in human saliva and breast milk[J]. Appl Environ Microbiol,2008,74(14):4434-4439.
[16] Vanderkelen L,Van Herreweghe J M,Callewaert L,et al. Goose-type lysozyme inhibitor (PliG) enhances survival of Escherichia coli in goose egg albumen[J]. Appl Environ Microbiol,2011, 77(13):4697-4699.
[17] Liu Z,García-Díaz B,Catacchio B,et al. Protecting gram-negative bacterial cell envelopes from human lysozyme:Interactions with Ivy inhibitor proteins from Escherichia coli and Pseudomonas aeruginosa[J]. Biochim Biophys Acta,2015,1848(11):3032-3046.

Antigen Epitope Peptides and Immunogenicity Analysis of E. coli O157: H7 Ivy Protein

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