绵羊痘病毒固原株L2R基因的克隆与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2017.01.028

Abstract

Abstract:

To explore the molecular characteristics of protein L2R from sheeppox virus(SPPV), genomic DNA was extracted from SPPV GY strain. The specific primers were designed and used to amplify the L2R gene from the genomic DNA by PCR. Then the PCR product was ligated into pGEM-T Easy vector. After transformation into E. coli Trans 5α, the positive clones were sequenced and the sequences were analyzed by the bioinformatic softwares. The result showed that L2R gene sequence contained an open reading frame (ORF) of 279 nucleotides and deduced protein consisted of 92 amino acids with the theoretical molecular weight of 10.92 ku and isoelectric point was 6.56. Analysis of secondary structure of protein L2R revealed that α-helix, β-strand, random coil and extended strand were 69.57%,9.78%,8.70% and 11.96%,respectively. Analysis of multiple sequence alignment showed that L2R gene from different capripox virus isolates were highly conserved, phylogenetic analysis showed that GY and NK, TU and SA was in a branch, indicating with a close genetic relationship among them. The present results laid a foundation for further studies of biological functions of protein L2R and interaction among the early proteins of capripox virus.

Key words: capripox virus; L2R gene; gene cloning; bioinformatics

CLC Number:

S852.65⁺4

LI Yang, YAN Xin-min, WU Guo-hua, LI Jian, YE Yi-you, ZHAO Zhi-xun, ZHU Hai-xia, ZHANG Qiang. Cloning and Bioinformatics Analysis of L2R Gene of Sheeppox Virus GY Strain[J]. , 2017, 44(1): 201-207.

References

[1] Rao T V, Bandyopadhyay S K. A comprehensive review of goatpox and sheeppox and their diagnosis[J]. Animal Health Research Review,2000,1(2):127-136.
[2] Murray M, Martin W B, Köylü A. Experimental sheep pox. A histological and ultrastructural study[J]. Research in Veterinary Science,1973,15(2):201-208.
[3] 颜新敏, 吴国华, 李健,等. 羊痘在中国的流行现状分析[J]. 中国农学通报, 2010, 26(24):6-9.
[4] 颜新敏, 张强. 羊痘病毒免疫学研究进展[J]. 动物医学进展, 2009, 30(12):67-70.
[5] Bhanuprakash V, Indrani B K, Hosamani M, et al. The current status of sheep pox disease[J]. Comparative Immunology Microbiology & Infectious Diseases, 2006, 29(1):27-60.
[6] 向智龙, 程振涛, 卓建华,等. 山羊痘病毒和羊口疮病毒双重PCR检测方法的建立与初步应用[J]. 中国畜牧兽医, 2011, 38(1):88-91.
[7] 陈轶霞, 才学鹏. 羊痘病毒分子特征及检测方法研究进展[J]. 畜牧与兽医, 2008, 40(11):96-99.
[8] 郝宝成, 梁剑平, 王学红,等. 山羊痘的流行及防治措施[J]. 中国畜牧兽医, 2011, 38(5):152-154.
[9] 朱学亮, 张强, 杨帆,等. 羊痘病毒载体研究进展[J]. 中国畜牧兽医, 2009, 36(5):98-101.
[10] Betakova T, Wolffe E J, Moss B. Regulation of vaccinia virus morphogenesis:Phosphorylation of the A14L and A17L membrane proteins and C-terminal truncation of the A17L protein are dependent on the F10L kinase[J]. Journal of Virology, 1999, 73(5):3534-3543.
[11] Wu X, Meng X, Yan B, et al. Vaccinia virus virion membrane biogenesis protein A11 associates with viral membranes in a manner that requires the expression of another membrane biogenesis protein, A6[J]. Journal of Virology, 2012, 86(20):11276-11286.
[12] Resch W, Weisberg A S, Moss A B. Vaccinia virus nonstructural protein encoded by the A11R gene is required for formation of the virionmembrane[J]. Journal of Virology, 2005, 79(11):6598-6609.
[13] Maruriavidal L, Weisberg A S, Moss B. Vaccinia virus L2 protein associates with the endoplasmic reticulum near the growing edge of crescent precursors of immature virions and stabilizes a subset of viral membrane proteins[J]. Journal of Virology, 2011, 85(23):12431-12441.
[14] Meng X, Embry A, Rose L, et al. Vaccinia virus A6 is essential for virion membrane biogenesis and localization of virion membrane proteins to sites of virionassembly[J]. Journal of Virology, 2012, 86(10):5603-5613.
[15] 王曼, 叶奕优, 赵志荀,等. 绵羊痘病毒固原株的分离与鉴定[J]. 中国兽医科学, 2015,45(4):402-406.
[16] Zeng X, Chi X, Li W, et al. Complete genome sequence analysis of goatpox virus isolated from China shows high variation[J]. Veterinary Microbiology, 2014, 173(1):38-49.
[17] Maruri-Avidal L, Domi A, Weisberg A S, et al. Participation of vaccinia virus L2 protein in the formation of crescent membranes and immature virions[J]. Clays & Clay Minerals, 2011, 85(6):2504-2511.
[18] 卢昌,吴国华,王曼,等.痘病毒入侵融合复合物的研究进展[J].动物医学进展,2014,35(9):97-101.
[19] Nelson G E, Wagenaar T R, Moss B. A conserved sequence within the H2 subunit of the vaccinia virus entry/fusion complex is important for interaction with the A28 subunit and infectivity[J]. Journal of Virology, 2008, 82(13):6244-6250.

Cloning and Bioinformatics Analysis of L2R Gene of Sheeppox Virus GY Strain

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