米托蒽醌通过活性氧诱导大鼠肝癌RH35细胞凋亡的研究

doi:10.16431/j.cnki.1671-7236.2016.12.024

Abstract

Abstract:

The assay was aimed to study the cytotoxicity and mechanisms of mitoxantrone (MTN) in rat hepatama cell line RH35. MTT assay was performed to assess the cytotoxicity of MTN, and microscope was used to observe cellular morphologic changes. Apoptotic ratio and intracellular ROS generation were measured by flow cytometric analysis. The protein expression was examined by Western blotting analysis. The results showed that MTN inhibited RH35 cell growth in a time-and concentration-dependent manner. The morphologic changes were observed in the cells, including cell shrinkage, membrane blebbing and apoptotic bodies when treated with 15 μmol/L MTN for 24 and 48 h. And MTN also induced the increase of apoptotic ratio and generation of ROS in a time dependent manner. In addition, the intracellular ROS generation ratio and the apoptotic ratio of MTN treated group were extremely decreased by the ROS scavenger NAC (P<0.01). The pretreatment of RH35 cells with 15 mmol/L NAC thoroughly reversed the MTN-induced enhancement of caspase-3, Bax and CytC level and attenuation of Bcl-2 level. In conclusion, MTN induced apoptosis in RH35 cells through increasing the generation of ROS.

Key words: mitoxantrone; apoptosis; RH35 cell; ROS

CLC Number:

Q813.5

ZHANG Ya-hong, GAN Ying, QI Jian-guo, WANG Jian-hong. Mitoxantrone Induced Apoptosis through ROS in Rat Hepatama RH35 Cell Line[J]. , 2016, 43(12): 3245-3250.

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Mitoxantrone Induced Apoptosis through ROS in Rat Hepatama RH35 Cell Line

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