猪圆环病毒2型山西株全基因组序列分析

doi:10.16431/j.cnki.1671-7236.2016.12.007

Abstract

Abstract:

In order to study genetic variation of porcine circovirus type 2 (PCV2) strains in Shanxi province, the genomic sequences of four PCV2 strains which were SXXZ1, SXXZ2, SXTY and SXJZ were isolated recently from some areas of Shanxi province, they were amplified, cloned and sequenced. The amplified PCV2 genomic sequences of these four strains were analyzed and compared with that of 34 published PCV2 stains by DNAStar and drawing phylogenetic tree. The results showed that the genomic sequences of SXJZ PCV2 strain was 1 768 bp, and the others were 1 767 bp, which accounted for 25% and 75%, respectively. The homologies of nucleotide sequences of the four strains were 95.9% to 100.0%, the homologies of nucleotide sequences of the four strains with the 34 isolates from different regions of the world PCV strain were 94.5% to 99.9%, and the homologies of nucleotide sequences of the four strains with the domestic vaccine strains were 95.6% to 99.8%. The phylogenetic tree analysis showed that SXXZ1, SXXZ2 and SXTY belonged to PCV-2b genotype, and SXJZ belonged to PCV-2e genotype. The evolutionary relationship among SXXZ1, SXXZ2 and the QY strain of Guangdong were closer, we also found a recent evolutionary relationship between SXTY and AUT5 of Austria, and a recent evolutionary relationship between SXJZ and FJ of Fujian. Besides the evolutionary relationship among SXXZ1, SXXZ2, SXTY and the DBN-SX07-2 of PCV2 vaccine of Shanxi province, a recent evolutionary relationship between SXJZ and LG of PCV2 vaccine were found. Thus PCV2 strains were popular based on PCV-2b in Shanxi province, also PCV-2e was isolated and existed. It laid a foundation for the molecular epidemiology, genetic variation, prevention and control of PCV2 in Shanxi province.

Key words: porcine circovirus type 2; genome; sequence analysis

CLC Number:

S858.28

MENG Fan, WU Xin, YAO Jing-ming, FAN Zhen-hua, LIU Wen-jun, WANG Juan-ping, HAN Yi-chao, XUE Yi-peng, MI Rui-juan, Li Yu-bin. Genome Sequence Analysis of Porcine Circovirus Type 2 Strains from Shanxi Province[J]. , 2016, 43(12): 3127-3134.

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Genome Sequence Analysis of Porcine Circovirus Type 2 Strains from Shanxi Province

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