Nsp9基因在Marc-145细胞上对猪繁殖与呼吸综合征病毒复制的影响

doi:10.16431/j.cnki.1671-7236.2016.12.005

Abstract

Abstract:

In order to evaluate whether Nsp9 could enhance the replication of porcine reproductive and respiratory syndrome virus (PRRSV), pIRES2-EGFP-Nsp9 plasmid containing whole Nsp9 genome were transfected into Marc-145 cells,Real-time PCR and Western blotting were used to evaluate the expression of N protein after PRRSV was inoculated. The results showed that the level of N protein in the Nsp9 transfected cells was significantly higher than control group (P<0.05),which was 1.5 times higher. Meanwhile,the expression at protein level had the same trend as the mRNA level. When the dose of plasmid was increasing,the expression of N protein both at the mRNA and protein levels were increased. In conclusion,Nsp9 gene could enhance the replication of PRRSV in Marc-145 cells.

Key words: PRRSV; Nsp9 gene; Marc-145 cell; replication

CLC Number:

Q785

ZHAO Meng-meng, FENG Song-lin, WANG Wen-jia, XING Xing, FENG Jia-ping, ZHANG Gui-hong. Effect of Nsp9 Gene on Replication of PRRSV in Marc-145 Cells[J]. , 2016, 43(12): 3114-3120.

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Effect of Nsp9 Gene on Replication of PRRSV in Marc-145 Cells

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