活性干酵母与酵母培养物对体外瘤胃发酵参数影响的比较

doi:10.16431/j.cnki.1671-7236.2016.11.018

Abstract

Abstract:

This study was conducted to compare the effect of active dry yeast（ADY)and yeast culture（YC）on gas production,dynamics of gas production,methane content and rumen fermentation parameters by in vitro gas production technique,and the values of ME,OMD and yield of MP were also predicted according to 24 h gas production,in order to further reveal the mechanism of the difference effect and lay a foundation for those reasonable application.The results showed that ADY and YC had similiar effect on gas production, however,the gas production of YC group at 12,24 and 48 h were higher than that of ADY;Compared to CON group,the rate of gas production in earlier fermentation period was increased by 19.4% in YC group and decreased by 15.3% in ADY group（P>0.05),and that of YC group was significantly increased by 41.0% than that of ADY group（P<0.05);The content of CH₄ of YC group was significantly increased and content of H₂ and CO₂ were significantly decreased（P<0.05).The content of H₂ in ADY group was significantly decreased（P<0.05),while the content of CH₄ and CO₂ were not significantly changed（P>0.05);The pH and total VFA of rumen fermentation fluid in ADY and YC groups were not significantly changed（P>0.05),while the concentration of NH₃-N of the two groups was significantly increased（P<0.05).ADY had no significant effect on individual VFA（P>0.05),whereas YC tended to increase the ratio of acetic acid and decrease the ratio of isobutyric acid;Comparing to CON group,the OMD,ME and MP of ADY and YC groups were not significantly affected（P>0.05), while that of YC group were all significantly increased than ADY group（P<0.05).In conclusion,there were certain differences in the effect of ADY and YC on gas production,concentrate of gas,the rate of gas production and parameter of rumen fermentation.

Key words: yeast preparation; in vitro gas production technique; gas production; rumen fermentation

CLC Number:

S816.6

GENG Chun-yin, ZHAO Li-ping, HE Li-wen, ZHOU Zhen-ming, MENG Qing-xiang. Comparison of the Effects of Active Dry Yeast and Yeast Culture on Rumen Fermentation Parameters[J]. , 2016, 43(11): 2931-2938.

References

[1] 耿春银,任丽萍,周振明,等.反刍动物酵母菌制剂应用的效果及可能作用机制[J].动物营养学报,2015,27(4):1011-1020.
[2] Denev S A,Peeva T Z,Radulova P,et al.Yeast cultures in ruminant nutrition[J].Bulgarian Journal of Agricultural Science,2007,13:357-374.
[3] 中华人民共和国农业部.NY/T 815-2004肉牛饲养标准[S].北京:中国农业出版社,2004.
[4] Menke K H,Steingass H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28(5):47-55.
[5] Ørskov E R,McDonald I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].The Journal of Agricultural Science,1979,92(2):499-503.
[6] Broderick G A,Kang J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.
[7] Li Y L,Meng Q X.Effect of different types of fibre supplemented with sunflower oil on ruminal fermentation and production of conjugated linoleic acids in vitro[J].Archives Animal Nutrition,2006,60(5):402-411.
[8] Menke K H,Raab L,Salewski A,et al.The estimation of digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro[J]. Journal of Agricultural Science,1979,93(1):217-222.
[9] Ali K H M.Influence of Saccharomyces cerevisiae on fermentation parameters and nutrient degradability in an in vitro study[D].Wien:University of Veterinary Medicine Vienna,2012.
[10] Wallace A J,Wallace S J A,Mckain N,et al.Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass by mixed ruminal microorganisms in vitro[J].Journal of Animal Science,2000,79(7):1905-1916.
[11] 班志彬,张国梁,杨华明.等.活性干酵母和纤维素酶对草原红牛瘤胃挥发性脂肪酸浓度及甲烷排放的影响[J],中国畜牧兽医,2013,40(2):57-61.
[12] Lila Z A,Mohammed N,Yasui T,et al.Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro[J].Journal of Animal Science,2004,82(6):1847-1854.
[13] 乔国华,单安山.直接饲喂微生物培养物对奶牛瘤胃发酵产甲烷及生产性能的影响[J].中国畜牧兽医,2006,33(5):11-14.
[14] Chaucheyras F,Fonty G,Bertin G,et al.In vitro H2 utilization by a ruminal acetogenic bacterium cultivated alone or in association with an archaea methanogen is stimulated by a probiotic strain of Saccharomyces cerevesiae[J].Applied Environmental Microbiology,1995,61(9):3466-3467.
[15] 郭嫣秋,胡伟莲,刘建新.瘤胃甲烷菌及甲烷生成的调控[J].微生物学报,2005,45(1):145-148.
[16] Thrune M,Bach A,Ruiz-Moreno M,et al.Effects of Saccharomyces cerevisiae on ruminal pH and microbial fermentation in dairy cows yeast supplementation on rumen fermentation[J].Livestock Science,2009,124(1):261-265.
[17] Kowalik B,Skomial J,Pajak J J,et al.Population of ciliates,rumen fermentation indicators and biochemical parameters of blood serum in heifers fed diets supplemented with yeast（Saccharomyces cerevisiae）preparation[J].Animal Science Papers and Reports,2012,30(4):329-338.
[18] Vyas D等著,孙鹏译.活性干酵母和灭活干酵母对肉用小母牛亚急性瘤胃酸中毒、瘤胃发酵及营养物质消化率的影响[J].中国畜牧兽医,2014,41(5):243.
[19] Hristov A N,Varga G,Cassidy T,et al.Effect of Saccharomyces cerevisiae fermentation product on ruminal fermentation and nutrient utilization in dairy cows[J].Journal of Dairy Science,2010,93(2):682-692.
[20] Mao H L,Mao H L,Wang J K,et al.Effects of Saccharomyces cerevisiae fermentation product on in vitro fermentation and microbial communities of low-quality forages and mixed diets[J].Journal of Animal Science,2013,91(7):3291-3298.
[21] Geng C Y,Ren L P,Zhou Z M,et al.Comparison of active dry yeast（Saccharomyces cerevisiae）and yeast culture for growth performance,carcass traits,meat quality and blood indexes in finishing cattle[J].Animal Science Journal,2016,87(8):982-988.
[22] Salvati G G S等著,赵连生译.夏季日粮中添加活性酵母对奶牛消化率和生产性能的影响[J].中国畜牧兽医,2015,42(8):2214.
[23] Oeztuerk H,Schroeder B,Beyerbach M,et al.Influence of living and autoclaved yeasts of saccharomyces boulardii on in vitro ruminal microbial metabolism[J].Journal of Dairy Science,2005,88(7):2594-2600.
[24] Richter G,Flachowsky G.Zum einsatz leistungssteigernder substanzen in der wiederkäuerernäh-rung-isosäuren und pansenfermoregulatoren monatsschr[J].Veterinary Medicine,1989,44:819-825.
[25] Yoon I K,Stern M D.Effects of Saccharomyces cerevisiae and Aspergillus oryzae on ruminal fermentation in dairy cows[J].Journal of Dairy Science,1996,79(3):411-417.
[26] Dawson K A,Neuman K E,Boling J A.Effects of microbial supplements containing yeast and Lactobacilli on roughage-fed ruminal microbial activities[J].Journal of Animal Science,1990,68(10):3392-3398.

Comparison of the Effects of Active Dry Yeast and Yeast Culture on Rumen Fermentation Parameters

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