不同浓度枯草芽孢杆菌对体外瘤胃发酵特性的影响

doi:10.16431/j.cnki.1671-7236.2019.04.009

Abstract

Abstract:

The in vitro gas production technique experiment with a single-factor design was conducted to compare the effect of the low-dose (T1,1.14×10¹⁰CFU/kg) and high-dose (T2,2.27×10¹⁰ CFU/kg) Bacillus subtilis on 12 and 48 h kinetic parameters of rumen gas production and rumen fermentation parameters of TMR (ratio of concentration and rough was 60:40),and the values of metabolizable energy (ME),organic matter digestibility (OMD)and yield of microbial protein (MCP) were also evaluated according to 24 h gas production.The purpose of the study was to provide a theoretical basis for Bacillus subtilis rational application in ruminants.The results indicated that both T1 and T2 had a significant effect (P<0.05) on 24 and 48 h gas production compared to control group (CON),and the gas production of T2 group was higher than that of T1 group (P<0.05).Moreover,T1 significantly increased the content of CH₄ compared to CON(P<0.05),while T2 had no significant effect on content of CH₄ (P>0.05).For rumen fermentation parameters,T2 increased the concentration of NH₃-N of 12 h (P<0.05),and had no significant effect on other fermentation indexes (P>0.05).Moreover,T1 decreased the content of butyric acid and valeric acid of 48 h (P<0.05),and T2 significantly reduced pH and increased the total volatile acid content (P<0.05).Both T1 and T2 had no significant effect on other indexes (P>0.05).In addition,the values of OMD,ME and MCP of T1 and T2 groups were significantly higher than those of CON,and the values of OMD and ME in T2 were significantly higher than those in T1 group (P<0.05).In conclusion,both low-and high-concentration Bacillus subtilis had a certain improvement effect on the fermentation parameters of the fermentation substrate (TMR,60:40),and high concentration (2.27×10¹⁰ CFU/kg) was better.

Key words: Bacillus subtilis; in vitro gas production technique; rumen fermentation

CLC Number:

S816.7

LI Yueming, LUAN Jiaming, ZHANG Min, JIN Yinghai, XIA Guangjun, GENG Chunyin. Effects of Bacillus subtilis on in vitro Rumen Fermentation Characteristics[J]. , 2019, 46(4): 1031-1037.

References

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Effects of Bacillus subtilis on in vitro Rumen Fermentation Characteristics

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