酵母诱导表达鸡IFN-α抗IBDV效果及其对信号分子PI3K和NF-κB的影响

doi:10.16431/j.cnki.1671-7236.2016.08.032

Abstract

Abstract:

To investigate the anti-IBDV activity of IFN-α and its effects on chicken lymphocyte signaling molecule PI3K and NF-κB activity,forty 10-day-old non-immune chickens were randomly separated into two groups:Blank control group and IFN-α group.The chicken of two groups were inoculated with IFN-α and saline via intramscular injection for three days,respectively.Then they were inoculated with IBDV.From 13 to 18-day-old,the lymphocytes were isolated from peripheral blood of chicken.The lymphocyte proliferation of chicken were detected by the MTT method,the PI3K and NF-κB p65 concentration in lymphocyte and the IBDV-Ag of serum were detected at different time with ELISA.The results showed that compared with the blank control group,IFN-α could stimulate chicken lymphocyte proliferation and the PI3K and NF-κB p65 concentration in chicken lymphocyte were extremely significantly increased before infection (P<0.01).The lymphocyte proliferation and the expression of PI3K and NF-κB p65 in cell after IBDV infection were extremely significantly higher than before (P<0.01).IFN-α could extremely significantly inhabit the proliferation of IBDV-Ag (P<0.01).IFN-α could significantly promote lymphocyte transformation by increasing PI3K concentration and the biological activity of lymphocyte transformation associated with the PI3K and NF-κB p65 expression,activation and nucleo-cytoplasmic transport.It also could reduce IBDV-Ag proliferation in chickens.The study built foundation for IFN-α protein immuno enhancement and anti-viral activity.

Key words: IFN-α; PI3K; nuclear factor; NF-κB p65; IBD

CLC Number:

S858.31

HAN Hai-feng, LI Yin-ju, CHENG Xiang-chao, ZHANG Chun-jie, YU Zu-hua, YANG Hui, YANG Rong-en, FU Wei. Study on the Anti-IBDV Activity of Chicken IFN-α and Its Effects on Signaling Molecule PI3K and NF-κB[J]. , 2016, 43(8): 2164-2169.

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Study on the Anti-IBDV Activity of Chicken IFN-α and Its Effects on Signaling Molecule PI3K and NF-κB

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