BRCA1基因多态性及其与奶牛乳房炎抗性的相关性研究

doi:10.16431/j.cnki.1671-7236.2015.12.031

Abstract

Abstract: In order to study mutations of bovine breast cancer 1 (BRCA1) gene and its association with somatic cell count and somatic cell score in Holstein dairy cows in Beijing.SNPs of BRCA1 gene exon 13 and 14 were obtained by cloning,sequence alignment and searching for known mutations.In our research,25025 T>A of exon 9 and 46126 G>T of exon 13 in BRCA1 were detected by SNaPshot in Holstein dairy cows raised on the outskirts of Beijing city,as well as the association between SNPs and somatic cell count (SCC) or somatic cell score (SCS) were also analysed.The results showed that both 25025 T>A and 46126 G>T were detected in Holstein dairy cows.TT genotype at locus 25025 T>A and GT genotype at locus 46126 G>T were dominant genotypes,respectively.At locus 25025 T>A,least squares mean of SCC and SCS in individuals with AA genotype was significantly lower than that with TT and TA genotypes (P<0.05;P<0.01).Least squares mean of SCC in individuals with TT genotype was significantly lower than that with GG and GT genotypes (P<0.05),while SCS of different genotypes showed no difference at locus 46126 G>T (P>0.05).These results preliminarily indicated that the 25025 T>A and 46126 G>T mutations of BRCA1 gene could be used in molecular marker-assisted selection programs of mastitis resistance in Holstein dairy cows.

Key words: Holstein dairy cows; BRCA1 gene; somatic cell count; somatic cell score

CLC Number:

S823.9⁺1

YANG Yu-ze, FENG Tao, TANG Shao-qing, ZHANG Yu-tao, JIN Yue-sheng, WANG Yan, ZHAO Chun-ying, LU Yong-qiang. Polymorphism of BRCA1 Gene and its Association with Mastitis Resistance in Holstein Dairy Cows[J]. , 2015, 42(12): 3323-3328.

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Polymorphism of BRCA1 Gene and its Association with Mastitis Resistance in Holstein Dairy Cows

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