营养限饲与补偿对蒙古羔羊体重、血清生化指标及胸脂脂肪因子基因表达的影响

doi:10.16431/j.cnki.1671-7236.2015.12.014

Abstract

Abstract: To study the effect of nutrient restriction and compensation on weight,serum indexes and gene expression of chest fat adipokine in Mongolian lamb,thirty male lambs were randomly divided into control group (20.26 kg± 3.63 kg) and feed restricted group (21.29 kg±3.00 kg).The experiment lasted for 120 days,including 60 days for feed restriction and 60 days for compensatory feed.At the end of each stage,five lambs from each group were slaughtered and chest fat was collected.Serum concentration of triglycerides,non-esterified fatty acids (NEFA),total cholesterol were measured.Quantitative Real-time PCR were used to determine Adiponectin,Visfatin,PPAR-γ and ACC genes expression.The results showed that:①The lamb body weight of restricted group kept maintain level and that of control group showed a increase tendency.During the compensatory period,the average daily gain (ADG) of restricted group was significantly higher than control group (P< 0.05).②During the restriction period,serum triglycerides concentration of lambs in restricted group was significantly decreased at day 30 and 60 (P< 0.05),and NEFA concentration was significantly increased at day 30 (P< 0.05).After 60 days of nutritional compensation,all serum parameters we tested were not significantly different between restrict group and control group (P >0.05).③During the restriction period,PPAR-γ and ACC gene expression in restricted group were significantly lower than that of control group (P< 0.05),while no significant differences were found during the compensation period (P>0.05).Adiponectin and Visfatin genes expression in chest fat was not significantly changed throughout the experiment (P >0.05).We could know that nutritional restriction reduced fatty acid synthesis and adipocyte differentiation ability,adipose tissue was mobilized energy metabolism,and total cholesterol was recoveried growth on the compensation.However,the ACC and PPAR-γ genes in restricted group were significantly decreased with the body weight increased rapidly during the compensatory stage.Adiponectin and Visfatin were not significantly different between restricted and compensatory stages.

Key words: Adiponectin; Visfatin; PPAR-γ; ACC; compensatory growth

CLC Number:

S826.8⁺2

LU Xin-xing, YANG Jin-li, WANG Hai-rong, WANG Gui-chao, GAO Ai-wu, ZHANG Li-min, HOU Xian-zhi. Effect of Nutrient Restriction and Compensation on Weight,Serum Indexes and Gene Expression of Chest Fat Adipokines in Mongolian Lamb[J]. , 2015, 42(12): 3202-3209.

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Effect of Nutrient Restriction and Compensation on Weight,Serum Indexes and Gene Expression of Chest Fat Adipokines in Mongolian Lamb

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