GPR54基因在绵羊不同组织中的表达及其在睾丸中的定位研究

doi:10.16431/j.cnki.1671-7236.2015.07.028

Abstract

Abstract: The objectives of this study were to evaluate the expression of G-protein coupled receptor 54 (GPR54) in various tissues and the cellular localization of testis of 4 to 6-month-old sheep.In this experiment,mRNA expression was detected by Real-time fluorescence quantitative PCR.Cellular localization of GPR54 in testis was examined by immunohistochemistry.The results showed that GPR54 mRNA was expressed in all the tissues,and abundantly expressed in hypothalamus,pituitary gland and testis.The expression level gradually increased with the individual growth and development,the mRNA and protein expression of GPR54 in testis of 6-month-old was significantly higher than 4-month-old and 5-month-old (P<0.05);It was detected that GPR54 expression only in spermatogonia and a very number of primary spermatocytes in the testis by immunohistochemical staining,and it could detect significantly that the GPR54 expression in many split early sperm cells.The results demonstrated that it had close relationship between GPR54 and sexually mature of animals and the process of spermatogenesis in male animals.

Key words: GPR54 gene; sheep; Real-time fluorescence quantitative PCR; immunohistochemical; testis

CLC Number:

S814.8

LU Pei-yao, WANG Fu-chuan, SONG Xian-yi, ZHANG Kai, ZHANG Ning, YUE Wen-bin. Expression of GPR54 Gene in Different Tissues and Cellular Localization in Testis of Sheep[J]. , 2015, 42(7): 1816-1822.

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Expression of GPR54 Gene in Different Tissues and Cellular Localization in Testis of Sheep

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