miR-142-3p对奶山羊乳腺上皮细胞泌乳功能的影响

doi:10.16431/j.cnki.1671-7236.2015.07.029

Abstract

Abstract: To determine the regulative role of miR-142-3p in lactation of dairy goat mammary epithelia cells (DGMECs),we selected the DGMECs in lactating dairy goat for material,used liposomal transfection techniques to silence miR-142-3p expression,and used Real-time quantitative PCR,Western blotting and Assay Kits to explore the changes of lactation after miR-142-3p inhibition.The results showed that when miR-142-3p was inhibited,the cell proliferation ability of DGMECs was increased,the secretion amount of β-casein and triglyceride were up-regulated.So we concluded that miR-142-3p could influence the lactation function of DGMECs,such as cell proliferation,β-casein and triglyceride secretion,by inhibiting the function of its target genes.

Key words: diary goat; mammary gland; miR-142-3p; cell proliferation; lactation

CLC Number:

S827

WANG Chu-yue, WANG Chun-mei, SHAO Li, ZHANG Li, LIN Ye, CUI Ying-jun, GAO Xue-jun, LI Qing-zhang. Effect of miR-142-3p on Lactation in Diary Goat Mammary Epithelial Cells[J]. , 2015, 42(7): 1823-1829.

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Effect of miR-142-3p on Lactation in Diary Goat Mammary Epithelial Cells

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