Abstract: The study was aimed to understand the relationship between the gene sequence of SRV₉ strain of oral rabies vaccine and biological characteristics,compare its major antigen site with other domestic and overseas strains of rabies vaccine,and clear the molecular basis as oral vaccine strain.The SRV₉ N,P,M,G and L genes were cloned by RT-PCR,and connected into the pMD19-T vector,respectively,identified by enzyme digestion,sequenced by DNAStar software,and compared its sequences and the main antigen sites with other 11 strains.The results showed that the sequence homologies of SRV₉ N,P,M,G and L genes with others were 81.8% to 100.0%,the whole genome phylogenetic tree showed that,all in the same big branch,they were belonged to genotypeⅠ,the evolutionary distance among SRV₉ and ERA,SAD B19,SAG-2 were near,and were far among SRV₉,aG and RC-HL.The amino acid homology analysis showed that the homology was high among SRV₉ and SAD B19,SAG-2,ERA in extramembrance,transmembrane and intramembrane regions.The results would provide experimental basis for the study of gene recombinant attenuated vaccine strain by molecular biology techniques and development of oral rabies vaccine.

Key words: rabies virus; SRV₉ strain; genome; sequence analysis