HPLC法同时测定复方吡喹酮浇泼剂中有效成分的含量

doi:10.16431/j.cnki.1671-7236.2015.04.028

Abstract

Abstract: The assay was aimed to establish the method to simultaneously determine praziquantel (PZQ) and eprinomectin (EPR) contents of compound praziquantel pour-on preparation by HPLC in single wavelengths. The chromatographic conditions were as follows, C18 column (250 mm×4.6 mm, 5 μm) with a column temperature of 30 ℃, the mobile phase was consisted of acetonitrile-water-trifluoroacetic acid (70:30:0.01) with a flow rate of 1.0 mL/min, and the detection wavelength was set at 245 nm. The results showed that the linear relation of PZQ was good in the linear range between 0.7506 and 7.5063 mg/mL, the linear relation of EPR was good in the linear range between 31.266 and 312.660 μg/mL, related coefficients were both 0.9999, and the average recovery was 99.0% with RSD 1.02% and 98.1% with RSD 0.32%, respectively. The established HPLC method was quick, simple, accurate and reliable, and could be applied to determine the PZQ and EPR contents of compound praziquantel pour-on preparation.

Key words: HPLC; pour-on preparation; praziquantel (PZQ); eprinomectin (EPR)

CLC Number:

S859.7

JIN Li-qin, CHEN Xiao-lan, LI Yong-Jun, YU Jie, JIANG Chun-mao. Simultaneous Determination of Effective Components of Compound Praziquantel Pour-on Preparation by HPLC[J]. , 2015, 42(4): 958-965.

References

[1] 王磊.吡喹酮蒸发透皮给药系统的研究及其应用[D].武汉:华中农业大学,2010.
[2] Hashem H,Ibrahim A E,Elhenawee M.A rapid stability indicating LC-method for determination of praziquantel in presence of its pharmacopoeial impurities[J].Arabian Journal of Chemistry, 2012,7:1-7.
[3] 申献玲,张洪.吡喹酮水凝胶栓剂在家兔体内的药动学研究[J].广东药学院学报,2006,22(1):25-31.
[4] 吴德智,马正,刘运锋,等.吡喹酮制剂的发展和应用[J].中国血吸虫病防治杂志,2013,25(2):194-196.
[5] 郑新生,郑新安,黄静,等.高浓度吡喹酮溶液的透皮吸收及对日本血吸虫病鼠的治疗试验[J].中国药学杂志,2007,42(6):450-453.
[6] 郑新生,姚宝安,王艳蕊,等.高浓度吡喹酮注射剂的研制及对小鼠日本血吸虫病的治疗试验[J].华中农业大学学报,2006,25(1):68-70.
[7] 陈小军,孙志良,杨惠麟.伊维菌素制剂在动物医学中的研究进展[J].湖南畜牧兽医,2005,12(1):1-3.
[8] 杨君宏,何继红,侯晓林,等.牛肌肉中阿维菌素类药物残留的免疫亲和色谱——高校液相色谱荧光检测方法的研究[J].中国畜牧兽医,2014,41(1):243-245.
[9] 潘保良,汪明,王玉万,等.埃普利诺菌素研究进展[J].中国兽医寄生虫病,2003,11(2):59-62.
[10] 蔡进忠,李春花,拉环,等.埃普利诺菌素浇泼剂对牦牛皮蝇幼虫驱除效果试验[J].青海畜牧兽医杂志,2009,39(5):10-12.
[11] 次素英,马丽娟,何双燕.RP-HPLC测定乙酰氨基阿维菌素的含量[J].药物分析杂志,2010,30(6):1129-1131.
[12] 曾振灵,陈杖榴,冯其辉.吡喹酮在猪体内的生物利用度及药物动力学研究[J].畜牧兽医学报,1993,24(2):170-173.
[13] 中国兽药典委员会.中华人民共和国兽药典(一部)(2010年版)[S].北京:中国农业出版社,2010.
[14] 于咏兰,汪明.RP-HPLC检测注射型复方驱虫剂中吡喹酮和伊维菌素的含量[J].中国兽医杂志,2010,1:92-94.
[15] Shah S R,Dey S,Pradhan P,et al.Method development and validation for simulataneous estimation of albendazole and praziquantel in bulk and in a synthetic mixture[J].Journal of Taibah University for Science,2014,8(1):54-63.

Simultaneous Determination of Effective Components of Compound Praziquantel Pour-on Preparation by HPLC

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