高产蛋白酶枯草芽孢杆菌JNB001产酶条件的优化

doi:10.16431/j.cnki.1671-7236.2015.02.028

Abstract

Abstract: This research was aimed to further improve the ability of protease produced by Bacillus subtilis JNB001.By series single factor and orthogonal analysis to optimize the enzyme-producing conditions from initial pH,fermentation temperature,inoculation volume,bottling volume and cell age,etc.The results showed that the optimum conditions were:Initial pH 7.0,inoculation volume of 7%,bottling volume of 35 mL in the 250 mL flask,cell age was 18 h,and cultured for 36 h at 35 ℃.Cultivated at these conditions,the protease activity of the strain JNB001 could reach to 371.66 U/mL.These consequences laid the foundation for the subsequent applications such as the design of harmless biological treatment tank,etc.

Key words: Bacillus subtilis; protease; fermentation conditions; optimization

CLC Number:

Q814

ZHU Li-bin, QU Dao-feng, SHI Chun-wei, HAN Jian-zhong, YU Quan-fa, WU Jin-song. Optimization of Fermentation Conditions on the Protease-producing Bacillus subtilis JNB001[J]. , 2015, 42(2): 414-419.

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Optimization of Fermentation Conditions on the Protease-producing Bacillus subtilis JNB001

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