沙冬青种子总黄酮提取纯化与急性毒性及抗氧化活性研究

doi:10.16431/j.cnki.1671-7236.2019.03.036

摘要/Abstract

摘要：

本研究旨在探讨沙冬青种子总黄酮的提取纯化、急性毒性和抗氧化活性等特性。采用超声辅助提取法提取纯化沙冬青种子总黄酮，用AlCl₃-CH₄O显色法进行沙冬青种子总黄酮含量测定；对纯化后沙冬青种子总黄酮进行急性毒性试验；采用体外Fenton体系产生羟基自由基（·OH）和邻苯三酚自氧化法产生超氧自由基（O₂^-），检测沙冬青种子总黄酮对·OH和O₂^-的清除作用；测定沙冬青种子总黄酮对衰老小鼠血清丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性的影响。结果显示，沙冬青种子总黄酮含量测定方法的精密度（RSD=0.78%）、重复性（RSD=0.52%）、稳定性（RSD=0.65%）、回收率（99.140%）均在误差范围内，提取纯化后沙冬青种子总黄酮含量达86.780%。沙冬青种子总黄酮属于无毒化合物，并对·OH和O₂^-具有良好的清除能力，在总黄酮作用剂量为1 250 μg/mL时，对·OH和O₂^-的清除率分别达96.45%和50.39%。与衰老模型组小鼠相比，沙冬青种子总黄酮可极显著提高小鼠血清SOD活力（P<0.01），其中，在150 mg/（kg·d）总黄酮剂量组，小鼠血清SOD活力高达102.42 U/mL。此外，与衰老模型组小鼠相比，沙冬青种子总黄酮可极显著降低小鼠血清中MDA含量（P<0.01），以150 mg/（kg·d）总黄酮剂量组降低效果最明显。上述结果提示，沙冬青种子总黄酮具有良好的自由基清除能力和抗氧化活性，从而有效改善和提高机体免疫力。

关键词: 沙冬青种子总黄酮; 提纯; 急性毒性; 自由基; 超氧化物歧化酶; 丙二醛

Abstract:

This experiment was conducted to explore the purification and the acute toxicity and the antioxidant activity of total flavonoids from the seeds of Ammopiptanthus.The total flavonoids from the seeds of Ammopiptanthus was extracted using ultrasonic-assisted extraction method.The content of total flavonoids from the seeds of Ammopiptanthus was measured with AlCl₃-CH₄O coloration method.The acute toxicity test of total flavonoids was evaluated in mice.The Fenton system of hydroxyl radicals (·OH) produced in vitro and the superoxide radicals (O₂^-) produced by pyrogallol autoxidation were used to determine the scavenging effect of total flavonoids on radicals.Besides,the effects of total flavonoids on serum malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in aging model mice were measured.The results showed that the precision (RSD=0.78%),reproducibility (RSD=0.52%),stability (RSD=0.65%) and recovery rate (99.140%) of the method for determination of total flavonoids from the seeds of Ammopiptanthus were in the range of error,and the contain of total flavonoids was 86.780%.The total flavonoids from the seeds of Ammopiptanthus belonged to a non-toxic medicine.The total flavonoids from the seeds of Ammopiptanthus had good scavenging ability to ·OH and O₂^-.In the group of 1 250 μg/mL,there were the best scavenging effects of ·OH and O₂^-,and the clearance rates reached 96.45% and 50.39%,respectively.The total flavonoids could extremely significantly increase the activity of SOD in the serum compared with aging model mice (P<0.01).In the group of 150 mg/(kg·d) total flavonoids,there was the most significant increase of the activity of SOD in the serum,and the activity reached 102.42 U/mL.Furthermore,the total flavonoids could extremely significantly reduce the content of MDA in the serum compared with aging model mice (P<0.01).In the group of 150 mg/(kg·d) total flavonoids,there was the most significant reduction in the content of MDA in the serum.The results suggested that the total flavonoids from the seeds of Ammopiptanthus had good antioxidant activity and scavenging ability on radicals and repair the immune function of aging model mice.

Key words: total flavonoids from the seeds of Ammopiptanthus; purification; acute toxicity; radicals; SOD; MDA

中图分类号:

S853.7

靳野, 梁鹏飞, 方梅, 张嘉男, 贾宁, 邓康, 黄伟宽. 沙冬青种子总黄酮提取纯化与急性毒性及抗氧化活性研究[J]. 《中国畜牧兽医》, 2019, 46(3): 940-948.

JIN Ye, LIANG Pengfei, FANG Mei, ZHANG Jianan, JIA Ning, DENG Kang, HUANG Weikuan. Extraction,Purification,Acute Toxicity and Antioxidant Activity of Total Flavonoids from Seeds of Ammopiptanthus[J]. , 2019, 46(3): 940-948.

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