褪黑素基础上补喂来曲唑和N-甲基-D-天冬氨酸对母羊血浆繁殖激素水平的影响

doi:10.16431/j.cnki.1671-7236.2018.12.018

摘要/Abstract

摘要：

试验旨在研究反季节给繁殖母羊补喂褪黑素（melatonin，MLT）及在MLT基础上补喂来曲唑（letrozole，LE）和N-甲基-D-天冬氨酸（N-methyl-D-aspartate，NMDA）对母羊血浆繁殖相关激素水平的影响，为调控母羊发情药物的开发及应用提供新思路。试验选取40只（3.0±0.5）岁经产萨福克母羊（平均体重为76.23 kg±12.25 kg）随机分为4组，每组10只，分别为对照组和试验Ⅰ、Ⅱ、Ⅲ组，分别饲喂基础日粮、基础日粮+MLT 10 mg/（只·d）、基础日粮+MLT 10 mg/（只·d）+LE 7 mg/（只·d）、基础日粮+MLT 10 mg/（只·d）+LE 7 mg/（只·d）+NMDA 300 mg/（只·d）。结果显示，与对照组相比，试验Ⅰ组母羊血浆中促卵泡素（FSH）、雌二醇（E₂）、雌三醇（E₃）及双氢睾酮（DHT）水平均极显著升高（P<0.01），睾酮（T）水平显著降低（P<0.05）；试验Ⅱ组，母羊血浆MLT和LH水平均极显著升高（P<0.01），FSH水平显著升高（P<0.05）；试验Ⅲ组，母羊血浆中MLT和促性腺激素释放激素（GnRH）水平均显著升高（P<0.05）。与试验Ⅰ组相比，试验Ⅱ组母羊血浆中MLT显著升高（P<0.05），LH和T水平均极显著升高（P<0.01）。试验Ⅲ组与试验Ⅰ、Ⅱ组相比，FSH水平极显著降低（P<0.01）。综上所述，MLT和LE对母羊繁殖相关激素的释放有促进作用，且两者结合使用可达到调控母羊繁殖相关激素水平的效果；MLT、LE和NMDA组合应用对母羊繁殖激素调控效果不佳。

关键词: 褪黑素; 来曲唑; N-甲基-D-天冬氨酸; 繁殖激素; 母羊

Abstract:

This experiment was conducted to study the effects of feeding melatonin (MLT) on the breeding ewes in the off-season and feeding letrozole (LE) and N-methyl-D-aspartate (NMDA) on the basis of MLT,and to provide reference for the development and utilization of regulating ewes estrus drugs.Forty health multiparous Suffolk ewes with the age of (3.0±0.5) years old and the average weight of 76.23 kg±12.25 kg were randomly divided into 4 groups,control group and test groups Ⅰ,Ⅱ and Ⅲ,10 in each group.Control group:Basal diet;Test groupⅠ:Basal diet+ MLT 10 mg/d;Test groupⅡ:Basal diet+MLT 10 mg/d+LE 7 mg/d;Test group Ⅲ:Basal diet+MLT 10 mg/d+LE 7 mg/d+NMDA 300 mg/d.The results showed that,compared with the control group,the levels of FSH,E₂,E₃ and DHT in ewes plasma extremely significantly increased (P<0.01),and the level of T significantly decreased (P<0.05) in test group Ⅰ;Plasma MLT and LH levels were extremely significantly increased (P<0.01) and the FSH level was significantly increased (P<0.05) in test groupⅡ;In test group Ⅲ,the levels of MLT and GnRH in the plasma of the ewe were significantly increased (P<0.05).Compared with test groupⅠ,the MLT in the plasma of test group Ⅱ was significantly increased (P<0.05),and the LH and T levels were extremely significantly increased (P<0.01).Compared with test groups Ⅰ and Ⅱ,the FSH level was extremely significantly lower in test group Ⅲ (P<0.01).In summary,MLT and LE had a promoting effect on the release of reproductive-related hormones in ewes,and the combination of the two could achieve the effect of regulating the hormone levels related to reproductive growth of ewes;The combined application of MLT,LE and NMDA had a poor effect on the regulation of reproductive hormones in ewes.

Key words: melatonin; letrozole; N-methyl-D-aspartate; reproductive hormones; ewe

中图分类号:

S826

赵国栋, 臧长江, 王根, 谢景龙, 杨健涛, 唐雪梅, 张娟丽, 杨开伦. 褪黑素基础上补喂来曲唑和N-甲基-D-天冬氨酸对母羊血浆繁殖激素水平的影响[J]. 《中国畜牧兽医》, 2018, 45(12): 3455-3462.

ZHAO Guodong, ZANG Changjiang, WANG Gen, XIE Jinglong, YANG Jiantao, TANG Xuemei, ZHANG Juanli, YANG Kailun. Effects of Supplement with Letrozole and N-methyl-D-aspartate on Plasma Reproductive Hormone Levels of Ewes on the Basis of Melatonin Supplementation[J]. , 2018, 45(12): 3455-3462.

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