TET介导的DNA主动去甲基化的机制与功能研究进展

doi:10.16431/j.cnki.1671-7236.2018.05.023

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (5): 1304-1311.doi: 10.16431/j.cnki.1671-7236.2018.05.023

TET介导的DNA主动去甲基化的机制与功能研究进展

李文, 曹俊国, 陈敏, 许保增

中国农业科学院特产研究所, 特种经济动物分子生物学重点实验室, 长春 130112

收稿日期:2017-10-16 出版日期:2018-05-20 发布日期:2018-05-19
通讯作者: 许保增 E-mail:xubaozeng@hotmail.com
作者简介:李文(1995-),女,河南洛阳人,硕士生,研究方向:特种经济动物遗传育种与繁殖,E-mail:liwen20012@yeah.net
基金资助:
国家自然科学基金面上项目（31772606）；吉林省国际科技合作项目（31772606）；吉林特研生物技术有限责任公司研发基金（20170015）；中国农业科学院创新工程（CAAS-ASTIP-2017-ISAPS）

Research Progress on Mechanism and Function of TET Mediated DNA Demethylation

LI Wen, CAO Junguo, CHEN Min, XU Baozeng

State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received:2017-10-16 Online:2018-05-20 Published:2018-05-19

摘要/Abstract

摘要：

TET蛋白是一种α-酮戊二酸/Fe²⁺依赖的双加氧酶家族，可以氧化5-甲基胞嘧啶（5mC）产生5-羟基甲基胞嘧啶（5hmC）、5-甲酰基胞嘧啶（5fC）和5-羧基胞嘧啶（5caC）。TET蛋白在DNA去甲基化过程中发挥关键作用，并参与哺乳动物早期发育过程。现在被广泛认可的一种途径是TET蛋白氧化5mC，接着由胸腺嘧啶糖苷酶（thymine DNA glycosylase，TDG）氧化5fC、5caC，且TDG更易切割5caC，最后经过碱基切除修复得到未被修饰的胞嘧啶，达到去甲基化的目的。去甲基化过程中调控方式主要包括调节TET蛋白水平和调节代谢产物及辅助因子。作者主要对胚胎发育前后去甲基化的作用进行了阐述。

关键词: TET蛋白; DNA去甲基化; 调控机制; 生殖作用

Abstract:

TET protein is a family of alpha-ketoglutarate/Fe²⁺ dependent dioxygenases that oxidize 5-methylcytosine (5mC) to produce 5-hydroxymethylcytosine (5hmC),5-formylcytosine (5fC) and 5-carboxycytosine (5caC).In recent years,TET protein play a key role in DNA demethylation,and involve in the early development of mammals.5mC was oxidized by TET protein,5fC and 5caC were oxidized by thymine DNA glycosylase (TDG),5caC was cut by TDG,and finally unmodified cytosine was obtained after base excision repair to achieve the purpose of demethylation,this pathway was widely accepted.Regulatory methods in demethylation include regulation of TET protein levels,regulation of metabolites and cofactors.And this article focuses on the role of demethylation during embryonic development.

Key words: TET proteins; DNA demethylation; regulatory mechanisms; reproductive effects

中图分类号:

李文, 曹俊国, 陈敏, 许保增. TET介导的DNA主动去甲基化的机制与功能研究进展[J]. 《中国畜牧兽医》, 2018, 45(5): 1304-1311.

LI Wen, CAO Junguo, CHEN Min, XU Baozeng. Research Progress on Mechanism and Function of TET Mediated DNA Demethylation[J]. , 2018, 45(5): 1304-1311.

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TET介导的DNA主动去甲基化的机制与功能研究进展

Research Progress on Mechanism and Function of TET Mediated DNA Demethylation

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