不同收集方法及添加半胱氨酸、肝素钠对黄牛卵母细胞体外成熟及发育的影响

doi:10.16431/j.cnki.1671-7236.2018.05.022

摘要/Abstract

摘要：

试验旨在研究不同卵母细胞收集方法及添加半胱氨酸、肝素钠对黄牛卵母细胞体外成熟及体外受精的影响。以黄牛为研究对象，采用两种方法（抽卵法和割卵法）抽取卵泡中的卵母细胞，比较两种方法获取的卵母细胞成熟率，结果发现，抽卵法获得的卵母细胞成熟率显著高于割卵法（P<0.05）。将获取的卵母细胞分为4组：A组（对照组，只添加基础成熟培养液）、B组（基础成熟培养液+200 μmol/L半胱氨酸）、C组（基础成熟培养液+20 μg/mL肝素钠）、D组（基础成熟培养液+200 μmol/L半胱氨酸+20 μg/mL肝素钠），结果发现，D组的卵母细胞成熟率显著高于A、B、C组（P<0.05），B、C组间卵母细胞成熟率无显著差异（P>0.05），但两组卵母细胞成熟率均显著高于A组（P<0.05）；A组卵母细胞卵裂率均显著低于B、C、D组（P<0.05），B、C、D组间卵母细胞卵裂率无显著差异（P>0.05）；D组囊胚率显著高于其他3组（P<0.05）。结果表明，抽卵法获得卵母细胞效率显著高于割卵法，肝素钠及半胱氨酸对黄牛卵母细胞体外成熟和体外受精都有促进作用，且同时添加两种物质对体外成熟的效果更佳。

关键词: 肝素钠; 半胱氨酸; 卵母细胞收集方法; 体外成熟; 体外受精

Abstract:

This study was aimed to examine the effects of different collection methods and supplementation of heparin and cysteine sodium on in vitro maturation and fertilization of bovine oocytes.In this experiment,bovine oocytes obtained from ovarian follicles were collected by two methods including the cutting and ovulation methods which were made comparison.The results showed that the mature rate of collected oocyte by ovulation method was significantly higher than cutting method (P<0.05).Furthermore,collected oocytes were divided into 4 groups:Group A (control group,maturation culture medium),group B (maturation culture medium+200 μmol/L cysteine),group C (maturation culture medium+20 μg/mL heparin sodium) and group D (maturation culture medium+200 μmol/L cysteine+20 μg/mL heparin sodium).The results showed that the maturation rate of oocytes in group D was significantly higher that groups A,B and C (P<0.05),there was no significant difference between groups B and C (P>0.05),but both of them were significantly higher than group A (P<0.05).The cleavage rate of oocytes in group A was significantly lower than groups B,C and D (P<0.05),but there was no significant difference in groups B,C and D (P>0.05).The blastocyst rate of group D was significantly higher than other three groups (P<0.05).In conclusion,this study results showed that ovulation method would be the better collecting method,supplementation of heparin sodium and cysteine on oocyte in vitro maturation and fertilization had a promoting effect,and in vitro maturation of adding two substances was better.

Key words: heparin sodium; cysteine; oocytes collection method; in vitro maturation; in vitro fertilization

中图分类号:

S814

夏威, 漆丹, 许晴, 叶思捷, 蒋涛. 不同收集方法及添加半胱氨酸、肝素钠对黄牛卵母细胞体外成熟及发育的影响[J]. 《中国畜牧兽医》, 2018, 45(5): 1297-1303.

XIA Wei, QI Dan, XU Qing, YE Sijie, JIANG Tao. Effects of Different Collection Methods and Supplementation of Heparin and Cysteine Sodium on in vitro Maturation and Fertilization of Bovine Oocytes[J]. , 2018, 45(5): 1297-1303.

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