藏红花素对小鼠卵母细胞体外成熟能力的影响

doi:10.16431/j.cnki.1671-7236.2021.10.025

摘要/Abstract

摘要： 试验旨在探讨藏红花素（crocin）的抗氧化应激作用对小鼠卵母细胞体外成熟及后续胚胎发育能力的影响。在体外成熟（IVM）培养液中添加不同浓度藏红花素（0、5、10、15、20、25、30 μmol/L），小鼠卵母细胞在体外成熟培养12 h后，检测卵母细胞第一极排出情况、卵母细胞胞质内活性氧（ROS）和谷胱甘肽（GSH）含量，并进行体外受精（IVF）；体外受精后6 h统计受精率，24 h统计卵裂率，96 h统计囊胚率。结果显示，与对照组相比，10、15 μmol/L藏红花素显著提高了卵母细胞第一极体排出率（P<0.05）；当藏红花素浓度继续增加时，卵母细胞的第一极体排出率下降，30 μmol/L藏红花素显著降低了卵母细胞第一极体排出率（P<0.05）；5、10、15 μmol/L藏红花素均显著降低了卵母细胞ROS含量（P<0.05），10、15 μmol/L藏红花素均显著提高了卵母细胞GSH含量（P<0.05）。与对照组相比，10 μmol/L藏红花素组受精率、卵裂率、囊胚率差异均不显著（P>0.05），15、20、25、30 μmol/L藏红花素均显著降低受精率和囊胚率（P<0.05），对卵裂率影响不显著（P>0.05）。结果表明，在小鼠卵母细胞体外成熟培养液中添加10 μmol/L藏红花素可以显著增加第一极体排出率，显著降低卵母细胞ROS含量、提高卵母细胞GSH含量，但对受精后的胚胎发育无显著影响。

关键词: 藏红花素; 卵母细胞; 体外成熟; 活性氧; 体外受精

Abstract: This study was aimed to investigate the effects of crocin on the in vitro maturation and subsequent embryonic development of mouse oocytes. Different concentrations of crocin (0, 5, 10, 15, 20, 25, 30 μmol/L) were added to the in vitro mature (IVM) culture medium, after IVM for 12 h, the first polar body extrution, the contents of reactive oxygen species (ROS) and glutathione (GSH) in the cytoplasm of oocytes were detected and then in vitro fertilization (IVF)was performed. Fertilization rate, cleavage rate and blastocyst rate were counted at 6, 24 and 96 h after IVF, respectively. The results showed that when compared with control group, the addition of 10 and 15 μmol/L crocin significantly increased the polar body extrution rate (P<0.05), and when the concentration of crocin further increased, the polar body extrution rate decreased, 30 μmol/L crocin significantly decreased the polar body extrution rate (P<0.05). 5, 10 and 15 μmol/L crocin could significantly reduce the content of ROS (P<0.05), and 10 and 15 μmol/L crocin increased the content of GSH of oocytes (P<0.05). Compared with control group, 10 μmol/L crocin had no significant difference in fertilization rate, cleavage rate and blastocyst rate (P>0.05), 15, 20, 25 and 30 μmol/L crocin all significantly decreased fertilization rate and blastocyst rate (P<0.05), and had no significant difference on cleavage rate (P>0.05). The results indicated that the addition of 10 μmol/L crocin to mouse oocytes in vitro maturation medium could significantly increase the rate of the first polar body extrution, reduce the content of ROS, and increase the content of GSH in oocytes, but did not affect the embryonic development ability of oocytes.

Key words: crocin; oocytes; in vitro maturation; reactive oxygen species; in vitro fertilization

中图分类号:

S823.8⁺3

艾日夏提·地里夏提, 周正娜, 张也, 刘志强, 章亚峰, 赵茜, 阿布力孜·吾斯曼. 藏红花素对小鼠卵母细胞体外成熟能力的影响[J]. 中国畜牧兽医, 2021, 48(10): 3734-3740.

AIRIXIATI Dilixiati, ZHOU Zhengna, ZHANG Ye, LIU Zhiqiang, ZHANG Yafeng, ZHAO Xi, ABULIZI Wusiman. Effects of Crocin on the in vitro Maturation and Embryonic Development of Mouse Oocytes[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3734-3740.

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