糖对牦牛卵母细胞体外成熟及其发育能力的影响

doi:10.16431/j.cnki.1671-7236.2017.01.021

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (1): 155-160.doi: 10.16431/j.cnki.1671-7236.2017.01.021

糖对牦牛卵母细胞体外成熟及其发育能力的影响

石仙¹, 熊显荣¹, 兰道亮², 胡嘉嘉¹, 蔡雯祎¹, 张大伟², 李键¹

1. 西南民族大学生命科学与技术学院, 成都 610041;
2. 西南民族大学青藏高原研究院, 成都 610041

收稿日期:2016-06-08 出版日期:2017-01-20 发布日期:2017-01-19
通讯作者: 李键 E-mail:lijian@swun.cn
作者简介:石仙(1990-),女,云南陆良人,硕士,研究方向:动物细胞与胚胎工程,E-mail:shixian_900824@163.com
基金资助:
四川省科技支撑计划（2014NZ0114）；西南民族大学研究生创新型科研项目（CX2016SZ076）

Effect of Sugar on in vitro Maturation and Developmental Competence of Yak Oocytes

SHI Xian¹, XIONG Xian-rong¹, LAN Dao-liang², HU Jia-jia¹, CAI Wen-yi¹, ZHANG Da-wei², LI Jian¹

1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;
2. College of Tibetan Plateau Research, Southwest University for Nationalities, Chengdu 610041, China

Received:2016-06-08 Online:2017-01-20 Published:2017-01-19

摘要/Abstract

摘要：

试验旨在研究不同种类、不同浓度的糖对牦牛卵母细胞体外成熟和发育能力的影响，进一步探索和优化牦牛卵母细胞培养体系，提高卵母细胞体外成熟和胚胎生产效率。在牦牛卵母细胞成熟液中添加不同浓度（0、5和10 mmol/L）的葡萄糖或蔗糖，培养24 h或预培养2 h后移入无糖培养基中继续培养22 h，统计卵母细胞体外成熟率及体外受精（IVF）后的胚胎卵裂率和囊胚率。结果显示，与对照组（0 mmol/L）相比，5和10 mmol/L葡萄糖组牦牛卵母细胞核成熟率和体外受精胚胎卵裂率均显著提高（P<0.05），10 mmol/L葡萄糖组的囊胚率最高，且与对照组相比差异显著（P<0.05）。添加10 mmol/L蔗糖可以显著提高牦牛卵母细胞核成熟率（P<0.05），但胚胎囊胚率与对照组相比差异不显著（P>0.05）。此外，用10 mmol/L葡萄糖预处理牦牛卵母细胞后其核成熟率、胚胎卵裂率和囊胚率最高，且均显著高于对照组（P<0.05）。由此可见，糖对牦牛卵母细胞体外成熟和发育有一定的影响，在成熟过程中添加适当浓度的糖能提高卵母细胞成熟率及体外受精胚胎发育能力。

关键词: 卵母细胞; 糖; 体外成熟; 牦牛

Abstract:

This study was designed to investigate the effect of different types and different concentrations of sugar on in vitro maturation(IVM) and developmental competence of yak oocytes, for being further research and optimization culture system of yak oocytes for efficient maturity yak oocytes and productivity of embryos. Immature yak oocytes were matured in vitro on culture medium with different concentrations (0,5 and 10 mmol/L) of glucose and sucrose in incubator for 24 h or 2 h pretreament with sugar and 22 h without sugar. Subsequently, then the maturation of oocytes,the cleavage rates and blastocyst formation rates after in vitro fertilization(IVF) were evaluated. The results showed that a medium with 5 and 10 mmol/L glucose IVM could significantly increase the yak oocytes maturation and cleavage (P<0.05), and the highest blastocyst formation rates in 10 mmol/L glucose group was significantly higher than 0 mmol/L glucose (P<0.05).10 mmol/L sucrose could increase significantly the nucleus maturation rates (P<0.05),and there was no significant difference of the blastocyst formation rates after IVF between 0 and 10 mmol/L sucrose (P>0.05). Furthermore, the nucleus maturation rates,IVF cleavage rates and blastocyst formation rates of yak oocytes which pretreated with 10 mmol/L glucose were the highest in these groups, and were higher than 0 mmol/L glucose (P<0.05). It manifested that the appropriate concentration of sugar could improve the quality of yak oocytes and embryos in vitro developmental competence, so it influenced in vitro development of yak oocytes indirectly.

Key words: oocyte; sugar; in vitro maturation; yak

中图分类号:

S814.6

石仙, 熊显荣, 兰道亮, 胡嘉嘉, 蔡雯祎, 张大伟, 李键. 糖对牦牛卵母细胞体外成熟及其发育能力的影响[J]. 《中国畜牧兽医》, 2017, 44(1): 155-160.

SHI Xian, XIONG Xian-rong, LAN Dao-liang, HU Jia-jia, CAI Wen-yi, ZHANG Da-wei, LI Jian. Effect of Sugar on in vitro Maturation and Developmental Competence of Yak Oocytes[J]. , 2017, 44(1): 155-160.

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糖对牦牛卵母细胞体外成熟及其发育能力的影响

Effect of Sugar on in vitro Maturation and Developmental Competence of Yak Oocytes

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