三穗麻鸭SOCS1基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2018.03.004

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (3): 590-597.doi: 10.16431/j.cnki.1671-7236.2018.03.004

三穗麻鸭SOCS1基因克隆及序列分析

龙立书¹, 华敏¹, 万润¹, 欧德渊^1,2, 苟万里³, 文明^1,2

1. 贵州大学动物科学学院, 贵阳 550025;
2. 贵州省动物疫病与兽医公共卫生重点实验室, 贵阳 550025;
3. 贵阳学院生物与环境工程学院, 贵阳 550005

收稿日期:2017-10-09 出版日期:2018-03-20 发布日期:2018-03-22
通讯作者: 欧德渊, 苟万里 E-mail:417996097@qq.com;381565046@qq.com
作者简介:龙立书(1993-),男,贵州锦屏人,硕士生,研究方向:动物病理学与组织胚胎学,E-mail:1393988581@qq.com
基金资助:
国家自然科学基金（31260607、31560703）；贵州省优秀青年科学人才培养计划项目（黔科合人字[2013]25号）；贵州省百层次创新型人才项目（黔科合人才[2016]4009号）；贵州省科技创新人才团队建设项目（黔科合人才团队[2015]4016号）；贵州大学研究生创新基金项目（研农2017008）

Cloning and Sequence Analysis of SOCS1 Gene in Sansui Duck

LONG Lishu¹, HUA Min¹, WAN Run¹, OU Deyuan^1,2, GOU Wanli³, WEN Ming^1,2

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Diseases and Veterinary Public Health in Guizhou Province, Guiyang 550025, China;
3. Department of Biology and Environment Engineering, Guiyang University, Guiyang 550005, China

Received:2017-10-09 Online:2018-03-20 Published:2018-03-22

摘要/Abstract

摘要：

为了分析三穗麻鸭细胞因子信号传导抑制蛋白（suppressors of cytokine signaling，SOCS）基因分子特征，预测其编码蛋白的生物学功能，本试验对三穗麻鸭SOCS1基因进行PCR扩增、克隆及序列测定，应用生物信息学方法对三穗麻鸭SOCS1基因进行序列分析，并对其编码蛋白的二级结构、保守结构域、跨膜结构域、信号肽和三级结构进行预测。结果显示，三穗麻鸭SOCS1基因全长为624 bp，可编码207个氨基酸；与大雁、原鸡、非洲爪蟾、猪、牛、人、大鼠和草鱼相应序列核苷酸序列同源性分别为97.6%、92.6%、68.3%、65.0%、64.8%、64.5%、63.5%和58.2%，氨基酸序列同源性分别为99.5%、96.6%、69.2%、64.0%、64.0%、67.9%、65.4%和50.8%；系统进化树显示，三穗麻鸭与大雁亲缘关系最近；编码蛋白的二级结构由无规则卷曲、α-螺旋、β-转角和延伸链组成，具有一个中央SH2区和SOCS盒，且无跨膜区和信号肽区域；三级结构呈弯曲螺旋结构。本试验结果为三穗麻鸭SOCS1基因编码蛋白的生物学功能研究奠定了理论基础。

关键词: 三穗麻鸭; SOCS1基因; 编码蛋白; 分子特征

Abstract:

In order to analyze the molecular characteristics of suppressors of cytokine signaling (SOCS) gene in Sansui duck,and predict the biological function of SOCS1 gene encoded protein,the SOCS1 gene was amplified,cloned and sequenced,the secondary structure,conserved domain analysis,transmembrane domain,signal peptide and tertiary structure of SOCS1 protein were predicted using bioinformatic methods.The results showed that the length of SOCS1 gene was 624 bp,encoding 207 amino acids.The SOCS1 gene of Sansui duck was shared a nucleotide identity of 97.6%,92.6%,68.3%,65.0%,64.8%,64.5%,63.5% and 58.2%,and an amino acid identity of 99.5%,96.6%,69.2%,64.0%,64.0%,67.9%,65.4% and 50.8% with those of Anser cygnoides,Gallus gallus,Xenopus laevis,Sus scrofa,Bos taurus,Homo sapiens,Rattus norvegicus and Ctenopharyngodon idella,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between Sansui duck and Anser cygnoides.The secondary structure of the encoding protein was based with random coil,alpha helix,beta turn and extended strand region,with a central SH2 domain and a SOCS box.There was no the transmembrane domains and the signal peptide,and with the curved spiral tertiary structure.These results provided a theoretical basis for the study of the biological function of SOCS1 gene encoding protein in Sansui duck.

Key words: Sansui duck; SOCS1 gene; encoding protein; molecular characterization

中图分类号:

Q785

龙立书, 华敏, 万润, 欧德渊, 苟万里, 文明. 三穗麻鸭SOCS1基因克隆及序列分析[J]. 《中国畜牧兽医》, 2018, 45(3): 590-597.

LONG Lishu, HUA Min, WAN Run, OU Deyuan, GOU Wanli, WEN Ming. Cloning and Sequence Analysis of SOCS1 Gene in Sansui Duck[J]. , 2018, 45(3): 590-597.

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三穗麻鸭SOCS1基因克隆及序列分析

Cloning and Sequence Analysis of SOCS1 Gene in Sansui Duck

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