基于RNA-Seq技术挖掘绵羊背最长肌肉质性状相关基因

doi:10.16431/j.cnki.1671-7236.2018.01.016

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (1): 122-130.doi: 10.16431/j.cnki.1671-7236.2018.01.016

基于RNA-Seq技术挖掘绵羊背最长肌肉质性状相关基因

王位^1,2, 付绍印^2,3,4, 何小龙³, 王艳欣^1,2, 王月星^1,2, 王标³, 刘斌^2,5, 刘永斌³, 张文广^1,2

1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
2. 动物遗传育种与繁殖内蒙古自治区重点实验室, 呼和浩特 010018;
3. 内蒙古自治区农牧业科学院, 呼和浩特 010031;
4. 内蒙古ATCG生物信息研究所, 呼和浩特 010020;
5. 内蒙古犇牛科技有限公司, 呼和浩特 010020

收稿日期:2017-06-28 出版日期:2018-01-20 发布日期:2018-01-20
通讯作者: 刘永斌, 张文广 E-mail:ybliu117@126.com;atcgnmbi@aliyun.com
作者简介:王位(1988-),男,内蒙古包头人,硕士生,研究方向:动物遗传育种与繁殖暨绵羊肉质特色基因挖掘,E-mail:ww282459824@126.com
基金资助:
国家自然科学基金项目（31360542）；内蒙古自治区农牧业科学院青年创新基金（2015QNJJM04）；国家肉羊产业技术体系建设项目（CARS-39）

Excavation of Meat Quality Related Genes in Longissimus Dorsi of Sheep by RNA-Seq

WANG Wei^1,2, FU Shaoyin^2,3,4, HE Xiaolong³, WANG Yanxin^1,2, WANG Yuexing^1,2, WANG Biao³, LIU Bin^2,5, LIU Yongbin³, ZHANG Wenguang^1,2

1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Key Laboratory of Animal Science, Breeding and Reproduction of the Inner Mongolia Autonomous Region, Hohhot 010018, China;
3. Inner Mongolia Academy of Agricultural & Animal Husbandry Science, Hohhot 010031, China;
4. Institute of ATCG, Nei Mongolia Bio-information, Hohhot 010020, China;
5. Nei Mongol BioNew Technology Co., Ltd., Hohhot 010020, China

Received:2017-06-28 Online:2018-01-20 Published:2018-01-20

摘要/Abstract

摘要：

试验通过对小尾寒羊与巴美肉羊背最长肌进行转录组分析，旨在挖掘影响两种绵羊肉质性状的关键基因。分别选取小尾寒羊和巴美肉羊各3只，利用转录组高通量测序（RNA-Seq）技术对其背最长肌转录组文库进行测序，利用生物信息学对测序结果进行分析，筛选影响两者肉质相关的候选基因。结果发现，6个样品测序数据经质量控制后共得到120 Gb的有效数据，共筛选出333个差异基因，其中上调基因有82个，下调基因有251个。GO功能富集与KEGG Pathway显著性富集分析结果共筛选了6个与肉质性状相关的候选基因：PDK4、MYF6、PPARGC1A、SLCO4A1、FABP4、LEP。试验通过对比小尾寒羊与巴美肉羊背最长肌转录组数据，筛选影响肉质性状的候选基因，丰富了绵羊基因组信息，为进一步阐述不同品种绵羊肉质性状分子机理奠定了基础。

关键词: RNA-Seq; 肉质性状; 转录组; 小尾寒羊; 巴美肉羊; 背最长肌

Abstract:

In order to obtain the genes related to meat quality traits, the transcriptome of longissimus dorsi of 3 Small-tail Han sheep and 3 Bamei mutton sheep were analyzed using RNA-Seq technology. The sequencing data was analyzed by bioinformatics methods then obtained the interrelated candidate genes which affect the meat quality traits. After quality control, 120 Gb clean data were obtained from 6 libraries. The differential expression gene were 333, which the expression of 82 genes was higher and 251 genes was lower. The results showed that 6 candidate genes (PDK4, MYF6, PPARGC1A, SLCO4A1, FABP4 and LEP) related to meat quality traits were screened by GO functional enrichment and KEGG Pathway analysis. Through comparison of the longissimus dorsi transcriptome data of Small-tail Han sheep and Bamei mutton sheep, we obtained the genes of meat quality traits, and enriched the information of sheep genome. This results laid a foundation for further elaboration of the molecular mechanism of meat quality traits in different sheep breeds.

Key words: RNA-Seq; meat quality trait; transcriptome; Small-tail Han sheep; Bamei mutton sheep; longissimus dorsi

中图分类号:

S813.3

王位, 付绍印, 何小龙, 王艳欣, 王月星, 王标, 刘斌, 刘永斌, 张文广. 基于RNA-Seq技术挖掘绵羊背最长肌肉质性状相关基因[J]. 《中国畜牧兽医》, 2018, 45(1): 122-130.

WANG Wei, FU Shaoyin, HE Xiaolong, WANG Yanxin, WANG Yuexing, WANG Biao, LIU Bin, LIU Yongbin, ZHANG Wenguang. Excavation of Meat Quality Related Genes in Longissimus Dorsi of Sheep by RNA-Seq[J]. , 2018, 45(1): 122-130.

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基于RNA-Seq技术挖掘绵羊背最长肌肉质性状相关基因

Excavation of Meat Quality Related Genes in Longissimus Dorsi of Sheep by RNA-Seq

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